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The Ca2+-binding protein calretinin is selectively enriched in a subpopulation of the epithelial rests of Malassez

Cell and Tissue Research volume 342, pages 391–400 (2010)Cite this article

Abstract

During tooth development, the inner and outer enamel epithelia fuse by mitotic activity to produce a bilayered epithelial sheath termed Hertwig’s epithelial root sheath (HERS). The epithelial rests of Malassez (ERM) are the developmental residues of HERS and remain in the adult periodontal ligament (PDL). Although the cellular regulation of the Ca2+-binding proteins parvalbumin, calbindin-D28k, and calretinin has been reported in the inner and outer enamel epithelia during tooth development, an involvement of Ca2+-binding proteins in the ERM has not so far been characterized. Among the three Ca2+-binding proteins tested (calbindin D28k, parvalbumin, calretinin), we have only been able to detect calretinin in a subpopulation of adult rat molar ERM, by using quantitative immunohistochemical and confocal immunofluorescence techniques. TrkA (a marker for ERM) is present in numerous epithelial cell clusters, whereas calretinin has been localized in the cytosol and perinuclear region of a subpopulation of TrkA-positive cells. We conclude that, in inner and outer enamel epithelial cells, Ca2+ is regulated by calbindin, parvalbumin, and calretinin during tooth development, whereas in the ERM of adult PDL, Ca2+ is regulated only by calretinin. The expression of Ca2+-binding proteins is restricted in a developmental manner in the ERM.

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Acknowledgments

The technical assistance of E. Janßen, J. Kozlowski, and Ch. Hoffmann is gratefully appreciated.

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Affiliations

  1. Department of Operative Dentistry, Periodontics and Endodontics, Heinrich Heine University, Moorenstrasse 5, 40225, Düsseldorf, Germany

    Yüksel Korkmaz, Thomas Beikler, Thorsten Blauhut, Kurt Schneider & Wolfgang H-M Raab

  2. Department I of Anatomy, University of Cologne, Cologne, Germany

    Franz-Josef Klinz & Klaus Addicks

  3. Department of Molecular and Cellular Sports Medicine, German Sports University, Cologne, Germany

    Wilhelm Bloch

Authors
  1. Yüksel Korkmaz
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  2. Franz-Josef Klinz
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  3. Thomas Beikler
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  4. Thorsten Blauhut
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  5. Kurt Schneider
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  6. Klaus Addicks
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  7. Wilhelm Bloch
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  8. Wolfgang H-M Raab
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Corresponding author

Correspondence to Yüksel Korkmaz.

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This study was supported by the Forschungskommission of the Heinrich Heine University of Düsseldorf.

The authors state no conflict of interest.

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Korkmaz, Y., Klinz, FJ., Beikler, T. et al. The Ca2+-binding protein calretinin is selectively enriched in a subpopulation of the epithelial rests of Malassez. Cell Tissue Res 342, 391–400 (2010). https://doi.org/10.1007/s00441-010-1076-3

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  • DOI: https://doi.org/10.1007/s00441-010-1076-3

Keywords

  • Epithelial rests of Malassez
  • Ca2+-binding proteins
  • Calretinin
  • Calbindin-D28k
  • Parvalbumin
  • Rat (Wistar)