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Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice

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Abstract

The periodontal ligaments (PDLs) are soft connective tissue between the cementum covering the tooth root surface and alveolar bone. PDLs are composed of collagen and elastic system fibers, blood vessels, nerves, and various types of cells. Elastic system fibers are generally formed by elastin and microfibrils, but PDLs are mainly composed of the latter. Compared with the well-known function of collagen fibers to support teeth, little is known about the role of elastic system fibers in PDLs. To clarify their role, we examined PDLs of mice underexpressing fibrillin-1 (mgR mice), which is one of the major microfibrillar proteins. The PDLs of homozygous mgR mice showed one-quarter of the elastic system fibers of wild-type (WT) mice. A close association between the elastic system fibers and the capillaries was noted in WT, homozygous and heterozygous mgR mice. Interestingly, capillaries in PDLs of homozygous mice were dilated or enlarged compared with those of WT mice. A comparable level of type I collagen, which is the major collagen in PDLs, was expressed in PDL-cells of mice with three genotypes. However, multi-oriented collagen fiber bundles with a thinner appearance were noted in homozygous mice, whereas well-organized collagen fiber bundles were seen in WT mice. Moreover, there was a marked decrease in periostin expression, which is known to regulate the fibrillogenesis and crosslinking of collagen. These observations suggest that the microfibrillar protein, fibrillin-1, is indispensable for normal tissue architecture and gene expression of PDLs.

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Acknowledgement

The authors are grateful to Professor Francesco Ramirez (Mount Sinai School of Medicine) for providing the mgR mutant mice. The authors would like to thank Dr. Kyoko Oka (Fukuoka Dental College) for valuable suggestions and technical advice.

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Authors and Affiliations

  1. Maxillofacial Orthognathics, Department of Maxillofacial Reconstruction and Function, Division of Maxillofacial/Neck Reconstruction, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8510, Japan

    Ganjargal Ganburged, Naoto Suda & Keiji Moriyama

  2. Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Diseases, Tokyo, Japan

    Ganjargal Ganburged, Naoto Suda & Keiji Moriyama

  3. Research Institute for Science and Technology, Tokyo University of Science, Chiba, Japan

    Masahiro Saito

  4. Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan

    Yosuke Yamazaki & Keitaro Isokawa

  5. Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan

    Yosuke Yamazaki & Keitaro Isokawa

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  1. Ganjargal Ganburged
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  2. Naoto Suda
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  3. Masahiro Saito
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  4. Yosuke Yamazaki
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  5. Keitaro Isokawa
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  6. Keiji Moriyama
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Correspondence to Naoto Suda.

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Funding

This study was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (No. 18390552, 19659547, and 21390546), by Sato Fund and Dental Research Center Grant at Nihon University School of Dentistry, and by Grant for Supporting Project for Strategic Research of Nihon University School Dentistry at Matsudo by the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Ganburged, G., Suda, N., Saito, M. et al. Dilated capillaries, disorganized collagen fibers and differential gene expression in periodontal ligaments of hypomorphic fibrillin-1 mice. Cell Tissue Res 341, 381–395 (2010). https://doi.org/10.1007/s00441-010-1021-5

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  • DOI: https://doi.org/10.1007/s00441-010-1021-5

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