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Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells

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Abstract

In situ hybridisation and immunohistochemistry analyses have shown that the Ciona intestinalis tumour necrosis factor alpha gene (CiTNFα), which has been previously cloned and sequenced, is expressed either during the inflammatory pharynx response to lipopolysaccharide (LPS) or during the swimming larval phase of development. Granulocytes with large granules and compartment/morula cells are CiTNFα-producing cells in both inflamed pharynx and larvae. Pharynx vessel endothelium also takes part in the inflammatory response. Haemocyte nodules in the vessel lumen or associated with the endothelium suggest the involvement of CiTNFα in recruiting lymphocyte-like cells and promoting the differentiation of inflammatory haemocytes. Specific antibodies against a CiTNFα peptide have identified a 43-kDa cell-bound form of the protein. Observations of pharynx histological sections (at 4 and 8 h post-LPS inoculation) from naive and medium-inoculated ascidians have confirmed the CiTNFα-positive tissue response. Larval histological sections and whole-mount preparations have revealed that CiTNFα is expressed by trunk mesenchyme, preoral lobe and tunic cells, indicating CiTNFα-expressing cell immigration events and an ontogenetic role.

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Authors and Affiliations

  1. Department of Animal Biology, Laboratory of Marine Immunobiology, University of Palermo, Via Archirafi 18, 90123, Palermo, Italy

    Nicolò Parrinello, Aiti Vizzini, Giuseppina Salerno, Maria Antonietta Sanfratello, Matteo Cammarata, Vincenzo Arizza, Mirella Vazzana & Daniela Parrinello

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  1. Nicolò Parrinello
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  2. Aiti Vizzini
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  3. Giuseppina Salerno
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  4. Maria Antonietta Sanfratello
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  5. Matteo Cammarata
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  6. Vincenzo Arizza
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  8. Daniela Parrinello
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Correspondence to Nicolò Parrinello.

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This work was supported by a research grant from the Italian Ministry of University and Scientific Research (PRIN 2006 to N. Parrinello).

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Parrinello, N., Vizzini, A., Salerno, G. et al. Inflamed adult pharynx tissues and swimming larva of Ciona intestinalis share CiTNFα-producing cells. Cell Tissue Res 341, 299–311 (2010). https://doi.org/10.1007/s00441-010-0993-5

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