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Proliferative cell types in embryonic lineages of the central complex of the grasshopper Schistocerca gregaria

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Abstract

The central complex of the grasshopper Schistocerca gregaria develops to completion during embryogenesis. A major cellular contribution to the central complex is from the w, x, y, z lineages of the pars intercerebralis, each of which comprises over 100 cells, making them by far the largest in the embryonic protocerebrum. Our focus has been to find a cellular mechanism that allows such a large number of cell progeny to be generated within a restricted period of time. Immunohistochemical visualization of the chromosomes of mitotically active cells has revealed an almost identical linear array of proliferative cells present simultaneously in each w, x, y, z lineage at 50% of embryogenesis. This array is maintained relatively unchanged until almost 70% of embryogenesis, after which mitotic activity declines and then ceases. The array is absent from smaller lineages of the protocerebrum not associated with the central complex. The proliferative cells are located apically to the zone of ganglion mother cells and amongst the progeny of the neuroblast. Comparisons of cell morphology, immunoreactivity (horseradish peroxidase, repo, Prospero), location in lineages and spindle orientation have allowed us to distinguish the proliferative cells in an array from neuroblasts, ganglion mother cells, neuronal progeny and glia. Our data are consistent with the proliferative cells being secondary (amplifying) progenitors and originating from a specific subtype of ganglion mother cell. We propose a model of the way that neuroblasts, ganglion mother cells and secondary progenitors together produce the large cell numbers found in central complex lineages.

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Acknowledgements

We are grateful to Dr. Yu Liu for critical comments on the manuscript, to Karin Fischer for excellent technical assistance and to M. Bastiani, G. Technau and the Developmental Studies Hybridoma Bank, Iowa for antibodies.

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  1. Developmental Neurobiology Group, Biozentrum, Ludwig-Maximilians-Universität München, Grosshadernerstrasse 2, 82152, Planegg-Martinsried, Germany

    George Boyan, Leslie Williams, Andrea Legl & Zsofia Herbert

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  1. George Boyan
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  2. Leslie Williams
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  3. Andrea Legl
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  4. Zsofia Herbert
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Correspondence to George Boyan.

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This work was supported by the Deutsche Forschungsgemeinschaft (grant: BO 1434/ 3-5).

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Boyan, G., Williams, L., Legl, A. et al. Proliferative cell types in embryonic lineages of the central complex of the grasshopper Schistocerca gregaria . Cell Tissue Res 341, 259–277 (2010). https://doi.org/10.1007/s00441-010-0992-6

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