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Developmental changes in β-subunit composition of Na,K-ATPase in the Drosophila eye

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Abstract

The Drosophila genome contains at least three loci for the Na,K-ATPase β-subunit; however, only the protein products of nrv1 and nrv2 have been characterized hitherto. Here, we provide evidence that nrv3 also encodes for a functional Na,K-ATPase β-subunit, as its protein product co-precipitates with the Na,K-ATPase α-subunit. Nrv3 expression in adult flies is restricted to the nervous system in which Nrv3 is enriched in selective types of sensory cells. Because Nrv3 expression is especially prominent in the compound eye, we have analyzed the subcellular and developmental distribution of Nrv3 within the visual cells and related this distribution to those of the α-subunit and of the β-subunits Nrv1 and Nrv2. Prospective visual cells express Nrv2 in the third larval instar stage and during the first half of pupal development. During the last third of pupal life, Nrv3 gradually replaces Nrv2 as the Na,K-ATPase β-subunit in the photoreceptor cells. Adult photoreceptors express Nrv3 as their major β-subunit; the visual cells R1–R6 co-express Nrv2 at a low level, whereas R7 and R8 co-express Nrv1. Notably, β-subunits do not co-distribute exactly with the α-subunit at some developmental stages, supporting the concept that the α-subunit and β-subunit can exist in the plasma membrane without being engaged in α/β heterodimers. The non-visual cells within the compound eye express almost exclusively Nrv2, which segregates together with the α-subunit to septate junctions throughout development.

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Acknowledgements

We are grateful to Stefan Baumgartner, Greg Beitel, and the Developmental Studies Hybridoma Bank (University of Iowa) for antibodies, to Lynn Cooley, Allan C. Spradling, and the Bloomington Stock Center for fly lines, to Bärbel Wuntke, Aya Sato, Hidenobu Ando, and Yosuke Ishida for experimental assistance, and the anonymous reviewers for their helpful comments.

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Authors and Affiliations

  1. Institute of Biochemistry and Biology, Department of Animal Physiology, University of Potsdam, Karl-Liebknecht-Strasse 24-25, 14476, Potsdam, Germany

    Otto Baumann

  2. Division of Neuroscience, Beckman Research Institute of the City of Hope, Duarte, CA, 91010, USA

    Paul M. Salvaterra

  3. Laboratory of Plasma Membrane and Nuclear Signaling, Graduate School of Biostudies, Kyoto University, Yoshida-Konoe-cho, Sakyo-ku, Kyoto, 606-8501, Japan

    Kunio Takeyasu

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  1. Otto Baumann
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Correspondence to Otto Baumann.

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Baumann, O., Salvaterra, P.M. & Takeyasu, K. Developmental changes in β-subunit composition of Na,K-ATPase in the Drosophila eye. Cell Tissue Res 340, 215–228 (2010). https://doi.org/10.1007/s00441-010-0948-x

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