Cell and Tissue Research

, Volume 338, Issue 2, pp 161–170 | Cite as

Novel conserved oligodendrocyte surface epitope identified by monoclonal antibody 4860

  • Tim Czopka
  • Eva Hennen
  • Alexander von Holst
  • Andreas Faissner
Regular Article

Abstract

Oligodendrocytes are the myelinating cells of the central nervous system. They differentiate from oligodendrocyte precursor cells through several intermediate states that can be followed by characteristic morphological changes and the expression of marker molecules. However, most oligodendrocyte lineage markers demarcate either the precursor or the differentiated oligodendrocyte in restricted subcellular compartments. Here, we describe a novel marker of the oligodendrocyte lineage recognised by the monoclonal antibody clone 4860. It selectively labels the surfaces of differentiated oligodendrocytes in culture and clearly differs from other oligodendrocyte markers. Importantly, the 4860 epitope highlights developing white matter tracts in rodent and avian brains and thus represents a useful and conserved feature. The 4860 epitope is not associated with protein backbones as revealed by the related 487/L5 antibody. Furthermore, the epitope disappears upon lipid extraction from cryosections or inhibition of sphingolipid synthesis in cultured oligodendrocytes. Thus, we conclude that mAb 4860 represents a novel lipid-based oligodendrocyte marker.

Keywords

Marker Oligodendrocyte precursor cells Myelin membrane Glycolipid Rodents 

References

  1. Bhat RV, Axt KJ, Fosnaugh JS, Smith KJ, Johnson KA, Hill DE, Kinzler KW, Baraban JM (1996) Expression of the APC tumor suppressor protein in oligodendroglia. Glia 17:169–174CrossRefPubMedGoogle Scholar
  2. Brockschnieder D, Sabanay H, Riethmacher D, Peles E (2006) Ermin, a myelinating oligodendrocyte-specific protein that regulates cell morphology. J Neurosci 26:757–762CrossRefPubMedGoogle Scholar
  3. Capela A, Temple S (2006) LeX is expressed by principle progenitor cells in the embryonic nervous system, is secreted into their environment and binds Wnt-1. Dev Biol 291:300–313CrossRefPubMedGoogle Scholar
  4. Czopka T, Von Holst A, Schmidt G, ffrench-Constant C, Faissner A (2009) Tenascin C and tenascin R similarly prevent the formation of myelin membranes in a RhoA-dependent manner, but antagonistically regulate the expression of myelin basic protein via a separate pathway. Glia (in press)Google Scholar
  5. Dugas JC, Tai YC, Speed TP, Ngai J, Barres BA (2006) Functional genomic analysis of oligodendrocyte differentiation. J Neurosci 26:10967–10983CrossRefPubMedGoogle Scholar
  6. Eisenbarth GS, Walsh FS, Nirenberg M (1979) Monoclonal antibody to a plasma membrane antigen of neurons. Proc Natl Acad Sci USA 76:4913–4917CrossRefPubMedGoogle Scholar
  7. Faissner A, Kruse J (1990) J1/tenascin is a repulsive substrate for central nervous system neurons. Neuron 5:627–637CrossRefPubMedGoogle Scholar
  8. Faissner A, Teplow DB, Kubler D, Keilhauer G, Kinzel V, Schachner M (1985) Biosynthesis and membrane topography of the neural cell adhesion molecule L1. EMBO J 4:3105–3113PubMedGoogle Scholar
  9. Faissner A, Clement A, Lochter A, Streit A, Mandl C, Schachner M (1994) Isolation of a neural chondroitin sulfate proteoglycan with neurite outgrowth promoting properties. J Cell Biol 126:783–799CrossRefPubMedGoogle Scholar
  10. Kessaris N, Fogarty M, Iannarelli P, Grist M, Wegner M, Richardson WD (2006) Competing waves of oligodendrocytes in the forebrain and postnatal elimination of an embryonic lineage. Nat Neurosci 9:173–179CrossRefPubMedGoogle Scholar
  11. Kippert A, Trajkovic K, Fitzner D, Opitz L, Simons M (2008) Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling. BMC Neurosci 9:40CrossRefPubMedGoogle Scholar
  12. Kruse J, Keilhauer G, Faissner A, Timpl R, Schachner M (1985) The J1 glycoprotein—a novel nervous system cell adhesion molecule of the L2/HNK-1 family. Nature 316:146–148CrossRefPubMedGoogle Scholar
  13. Levine JM, Stallcup WB (1987) Plasticity of developing cerebellar cells in vitro studied with antibodies against the NG2 antigen. J Neurosci 7:2721–2731PubMedGoogle Scholar
  14. Levine JM, Reynolds R, Fawcett JW (2001) The oligodendrocyte precursor cell in health and disease. Trends Neurosci 24:39–47CrossRefPubMedGoogle Scholar
  15. Ligon KL, Fancy SP, Franklin RJ, Rowitch DH (2006) Olig gene function in CNS development and disease. Glia 54:1–10CrossRefPubMedGoogle Scholar
  16. McCarthy KD, Vellis J de (1980) Preparation of separate astroglial and oligodendroglial cell cultures from rat cerebral tissue. J Cell Biol 85:890–902CrossRefPubMedGoogle Scholar
  17. Merrill AH Jr, Echten G van, Wang E, Sandhoff K (1993) Fumonisin B1 inhibits sphingosine (sphinganine) N-acyltransferase and de novo sphingolipid biosynthesis in cultured neurons in situ. J Biol Chem 268:27299–27306PubMedGoogle Scholar
  18. Poltorak M, Sadoul R, Keilhauer G, Landa C, Fahrig T, Schachner M (1987) Myelin-associated glycoprotein, a member of the L2/HNK-1 family of neural cell adhesion molecules, is involved in neuron-oligodendrocyte and oligodendrocyte-oligodendrocyte interaction. J Cell Biol 105:1893–1899CrossRefPubMedGoogle Scholar
  19. Pringle NP, Richardson WD (1993) A singularity of PDGF alpha-receptor expression in the dorsoventral axis of the neural tube may define the origin of the oligodendrocyte lineage. Development 117:525–533PubMedGoogle Scholar
  20. Raff MC, Miller RH, Noble M (1983) A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium. Nature 303:390–396CrossRefPubMedGoogle Scholar
  21. Ranscht B, Clapshaw PA, Price J, Noble M, Seifert W (1982) Development of oligodendrocytes and Schwann cells studied with a monoclonal antibody against galactocerebroside. Proc Natl Acad Sci USA 79:2709–2713CrossRefPubMedGoogle Scholar
  22. Rathjen FG, Schachner M (1984) Immunocytological and biochemical characterization of a new neuronal cell surface component (L1 antigen) which is involved in cell adhesion. EMBO J 3:1–10PubMedGoogle Scholar
  23. Reynolds BA, Tetzlaff W, Weiss S (1992) A multipotent EGF-responsive striatal embryonic progenitor cell produces neurons and astrocytes. J Neurosci 12:4565–4574PubMedGoogle Scholar
  24. Simons M, Trotter J (2007) Wrapping it up: the cell biology of myelination. Curr Opin Neurobiol 17:533–540CrossRefPubMedGoogle Scholar
  25. Sommer I, Schachner M (1981) Monoclonal antibodies (O1 to O4) to oligodendrocyte cell surfaces: an immunocytological study in the central nervous system. Dev Biol 83:311–327CrossRefPubMedGoogle Scholar
  26. Stallcup WB, Beasley L (1987) Bipotential glial precursor cells of the optic nerve express the NG2 proteoglycan. J Neurosci 7:2737–2744PubMedGoogle Scholar
  27. Streit A, Faissner A, Gehrig B, Schachner M (1990) Isolation and biochemical characterization of a neural proteoglycan expressing the L5 carbohydrate epitope. J Neurochem 55:1494–1506CrossRefPubMedGoogle Scholar
  28. Streit A, Yuen CT, Loveless RW, Lawson AM, Finne J, Schmitz B, Feizi T, Stern CD (1996) The Le(x) carbohydrate sequence is recognized by antibody to L5, a functional antigen in early neural development. J Neurochem 66:834–844PubMedCrossRefGoogle Scholar
  29. Terada N, Kidd GJ, Kinter M, Bjartmar C, Moran-Jones K, Trapp BD (2005) Beta IV tubulin is selectively expressed by oligodendrocytes in the central nervous system. Glia 50:212–222CrossRefPubMedGoogle Scholar
  30. von Holst A, Sirko S, Faissner A (2006) The unique 473HD-chondroitinsulfate epitope is expressed by radial glia and involved in neural precursor cell proliferation. J Neurosci 26:4082–4094CrossRefPubMedGoogle Scholar
  31. Wegner M, Stolt CC (2005) From stem cells to neurons and glia: a Soxist's view of neural development. Trends Neurosci 28:583–588CrossRefPubMedGoogle Scholar
  32. Yanagisawa M, Yu RK (2007) The expression and functions of glycoconjugates in neural stem cells. Glycobiology 17:57R–74RCrossRefPubMedGoogle Scholar
  33. Zhang SC (2001) Defining glial cells during CNS development. Nat Rev 2:840–843CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Tim Czopka
    • 1
    • 2
  • Eva Hennen
    • 1
    • 2
  • Alexander von Holst
    • 1
  • Andreas Faissner
    • 1
  1. 1.Department of Cell Morphology and Molecular NeurobiologyRuhr UniversityBochumGermany
  2. 2.International Graduate School of Neuroscience (IGSN)Ruhr UniversityBochumGermany

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