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Hyaluronan substratum holds mesenchymal stem cells in slow-cycling mode by prolonging G1 phase

Cell and Tissue Research volume 334, pages 435–443 (2008)Cite this article

Abstract

We examined, in vitro, whether hyaluronan induces slow cycling in placenta-derived mesenchymal stem cells (PDMSCs) by comparing cell growth on a hyaluronan-coated surface with cell growth on a tissue-culture polystyrene surface. The hyaluronan-coated surface significantly downregulated the proliferation of PDMSCs, more of which were maintained in the G0/G1 phases than were cells on the tissue-culture polystyrene surface. Both PKH-26 labeling and BrdU incorporation assays showed that most PDMSCs grown on a hyaluronan-coated surface duplicated during cultivation indicating that the hyaluronan-coated surface did not inhibit PDMSCs from entering the cell cycle. Mitotic synchronization showed that the G1-phase transit was prolonged in PDMSCs growing on a hyaluronan-coated surface. Increases in p27Kip1 and p130 were the crucial factors that allowed hyaluronan to lengthen the G1 phase. Thus, hyaluronan might be a promising candidate for maintaining stem cells in slow-cycling mode by prolonging their G1-phase transit.

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Affiliations

  1. Institute of Biotechnology and Center for Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan

    Chi-Mou Liu & Lynn L. H. Huang

  2. Department of Obstetrics and Gynecology, National Cheng Kung University, Tainan, Taiwan

    Chen-Hsiang Yu, Chiung-Hsin Chang & Chao-Chin Hsu

  3. Institute of Clinical Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan

    Lynn L. H. Huang

  4. Institute of Biotechnology, National Cheng Kung University, 1 University Road, Tainan, 70101, Taiwan

    Lynn L. H. Huang

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  1. Chi-Mou Liu
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  2. Chen-Hsiang Yu
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  3. Chiung-Hsin Chang
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  4. Chao-Chin Hsu
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  5. Lynn L. H. Huang
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Correspondence to Lynn L. H. Huang.

Additional information

This work was supported by research grant NSC95-2745-B-006-003-MY2 from the National Science Council, Taiwan, and by Landmark Project Grant A25, funded by the Taiwan Ministry of Education, from National Cheng Kung University.

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Liu, CM., Yu, CH., Chang, CH. et al. Hyaluronan substratum holds mesenchymal stem cells in slow-cycling mode by prolonging G1 phase. Cell Tissue Res 334, 435–443 (2008). https://doi.org/10.1007/s00441-008-0699-0

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  • DOI: https://doi.org/10.1007/s00441-008-0699-0

Keywords

  • Mesenchymal stem cells
  • Hyaluronan
  • Proliferation
  • Slow-cycling
  • Cell cycle