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Cellular localization of a putative Na+/H+ exchanger 3 during ontogeny in the pronephros and mesonephros of the Japanese black salamander (Hynobius nigrescens Stejneger)

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Abstract

The cloning of cDNA and an examination of the tissue distribution of Na+/H+ exchanger 3 (NHE3) were carried out in the Japanese black salamander, Hynobius nigrescens. The cellular localization of Hynobius NHE3 was examined by in situ hybridization and immunohistochemistry during ontogeny in the nephron of the pronephros and mesonephros of the salamander. The partial amino acid sequence of Hynobius NHE3 was 81% and 72% identical to rat NHE3 and stingray NHE3, respectively. Hynobius NHE3 mRNA and protein were exclusively expressed along the late portion of the distal tubule to the anterior part of the pronephric duct of premetamorphic larvae (IY stages 43–50). NHE3 mRNA was expressed in the pronephros but not in the external gills in the larvae at the digit differentiation stage (IY stage 50). In the adult, mRNA was strongly expressed in the mesonephros but not in the ventral and dorsal skin. In juvenile and adult specimens, NHE3 immunoreactivity was observed at the apical membrane of the initial parts of the distal tubules of the mesonephric kidney. Immunohistochemical and in situ hybridization studies suggested that Na+ absorption coupled with H+ secretion via NHE3 occurred in the distal nephron of the pronephros and mesonephros. This is the first study to indicate NHE3 expression during ontogeny in amphibians.

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Acknowledgements

We thank T. Nomura and T. Umebayashi for their kind help and suggestions for collecting animals. We are also grateful to Dr. K. Uchida and Prof. M. Nozaki of Niigata University for their valuable advice and assistance with regard to the in situ hybridization technique.

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Authors and Affiliations

  1. Department of Biological Science, Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama, 930-8555, Japan

    Tomoko Kumano, Norifumi Konno, Tatsuya Wakasugi, Kouhei Matsuda & Minoru Uchiyama

  2. Department of Biology, Matsumoto Dental University, 1780 Hirookagohara, Shiojiri, Nagano, 399-0781, Japan

    Hideki Yoshizawa

Authors
  1. Tomoko Kumano
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  2. Norifumi Konno
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  3. Tatsuya Wakasugi
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  4. Kouhei Matsuda
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  5. Hideki Yoshizawa
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  6. Minoru Uchiyama
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Corresponding author

Correspondence to Minoru Uchiyama.

Additional information

This work was supported in part by a research grant (a priority project in Science Faculty) from the University of Toyama to M.U.

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Kumano, T., Konno, N., Wakasugi, T. et al. Cellular localization of a putative Na+/H+ exchanger 3 during ontogeny in the pronephros and mesonephros of the Japanese black salamander (Hynobius nigrescens Stejneger). Cell Tissue Res 331, 675–685 (2008). https://doi.org/10.1007/s00441-007-0544-x

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  • DOI: https://doi.org/10.1007/s00441-007-0544-x

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