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Characteristics of resident dendritic cells in various regions of rat periodontal ligament

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Abstract

In the periodontal ligament (PDL) of normal rat molars, a considerable number of dendritic cells (DCs) are distributed as resident immunocompetent cells. We have hypothesized that the resident type of DCs can alter their morphology and phenotype to adapt to the microenvironment of the PDL. Thus, we have compared the characteristics of DCs in various regions of rat PDL by immunoelectron microscopy and gene expression analysis by means of reverse transcription/polymerase chain reaction. DCs in the periapical region show immature characteristics, i.e., small round profiles and few organelles. In the mesial region, which is the most stable area in the PDL, the density of DCs is the highest. DCs in the furcal region show the characteristics of activated antigen-presenting cells, i.e., CD86high expression, electron-dense lysosomes, and cell-to-cell contacts with lymphocytes. When the PDL is bacterially challenged by pulp exposure, the number of CD86+ DCs and contacts with lymphocytes increase in the furcal region. Gene expression analysis for the furcal region has also shown the up-regulation of the major histocompatibility complex class II alpha-chain, CD83, CD86, and the antigen-presenting cell-lectin-like receptor A1. Thus, we have demonstrated heterogeneity in resident DCs. This may be associated with the difference in DCs regarding their state of maturation/activation, which may further reflect microenvironmental differences such as the amount of external antigens.

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Acknowledgements

The authors thank Dr. Jacques E. Nör, Cariology, Restorative Sciences, and Endodontics, School of Dentistry, University of Michigan, for his technical advice regarding RT-PCR.

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Authors and Affiliations

  1. Pulp Biology and Endodontics, Graduate School, Tokyo Medical and Dental University, Yushima 1-5-45, Bunkyo-Ku, Tokyo, 113-8549, Japan

    Tomoatsu Kaneko, Reika Kaneko & Hideaki Suda

  2. Division of Cariology, Operative Dentistry and Endodontics, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Takashi Okiji

  3. Cariology, Restorative Sciences, and Endodontics, University of Michigan, Ann Arbor, Mich., USA

    Tomoatsu Kaneko

Authors
  1. Tomoatsu Kaneko
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  2. Takashi Okiji
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  3. Reika Kaneko
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  4. Hideaki Suda
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Corresponding author

Correspondence to Tomoatsu Kaneko.

Additional information

This study was supported by Grants-in-Aid for Scientific Research (nos. 17390508 and 19659496 to T.O. and no. 18791393 to T.K.) from the Japan Society for the Promotion of Sciences.

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Kaneko, T., Okiji, T., Kaneko, R. et al. Characteristics of resident dendritic cells in various regions of rat periodontal ligament. Cell Tissue Res 331, 413–421 (2008). https://doi.org/10.1007/s00441-007-0539-7

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  • DOI: https://doi.org/10.1007/s00441-007-0539-7

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