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Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells

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Abstract

We investigated porcine dental follicle cells at the early crown-formation stage and examined the behavior of cells grown in a collagen type I (Col-I) matrix. Clone-porcine dental follicle cells (DFC-I) and controls, viz., dental follicle itself, nonclone-dental follicle cells, periodontal ligament cells (PDLC), and bone marrow stromal cells, were obtained from 6-month-old pigs. DFC-I showed a different gene expression pattern from controls by reverse-transcription polymerase chain reaction analysis. In addition, Col-I treatment enhanced DFC-I proliferation and increased their alkaline phosphatase activity compared with nontreated DFC-I. The expression of periostin, biglycan, and osteocalcin (OCN) in cells growing on collagen was upregulated, similar to the pattern seen in PDLC. DFC-I with and without Col-I treatment were combined with β-tricalcium phosphate particles and implanted into immunodeficient mice. Significant differences were found in the gene expression patterns of bone sialoprotein, OCN, and periostin in both treated and non-treated implants at 2 and/or 4 weeks. The results showed that Col-I induced the mineralization pathway in these cells. Hard tissue formation was observed in both implant types at 8 weeks. Our results suggest that Col-I facilitates the differentiation of DFC-I along the mineralization process.

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Acknowledgments

We thank Dr. J. Sodek for generously providing the collagen type I and BSP antibodies (University of Toronto, Canada), Dr. H. Irie for the kind gift of the Osferion G1 (Olympus, Japan), and Dr. A. Kamiya (IMSUT, Japan ) for technical support.

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Authors and Affiliations

  1. Tooth Regeneration, The Division of Stem Cell Engineering, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shiroganedai, Minato-ku, Tokyo, 108-8639, Japan

    S. Tsuchiya, M. J. Honda, Y. Shinohara & M. Ueda

  2. Department of Oral and Maxillofacial Surgery, Nagoya University Postgraduate School of Medicine, 65 Tsurumacho, Showa-ku, Nagoya, Aichi, 466-8550, Japan

    S. Tsuchiya & M. Ueda

  3. Department of Operative Dentistry and Endodontics, Kanagawa Dental College, 82 Inaokacho, Yokosuka, Kanagawa, 238-8580, Japan

    M. Saito

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  1. S. Tsuchiya
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  2. M. J. Honda
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  3. Y. Shinohara
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  4. M. Saito
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  5. M. Ueda
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Correspondence to M. J. Honda.

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This work was supported in part by grants from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (Kakenhi Kiban B 16390578 and Houga 18659592 to M.J.H.) and by the Hitachi Medical Corporation (Japan) and DENICS International (Japan).

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Tsuchiya, S., Honda, M.J., Shinohara, Y. et al. Collagen type I matrix affects molecular and cellular behavior of purified porcine dental follicle cells. Cell Tissue Res 331, 447–459 (2008). https://doi.org/10.1007/s00441-007-0532-1

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  • DOI: https://doi.org/10.1007/s00441-007-0532-1

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