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Post-embryonic pericardial cells of Drosophila are required for overcoming toxic stress but not for cardiac function or adult development

Cell and Tissue Research volume 331, pages 565–570 (2008)Cite this article

Abstract

The Drosophila heart is composed of two cell types: cardioblasts (CB) and pericardial cells (PC). Whereas CBs act to maintain rhythmic contractions, the functions of accessory PCs are not clear. The close association between these two cell types has led to speculation of a cardio-regulatory role for PCs. However, we find that viability and cardiac function are normal in larvae following post-embryonic ablation of PCs by induced cell death. Removal of PCs during the larval instars or before metamorphosis results in viable and fertile adults. Interestingly, such animals have a reduced lifespan and increased sensitivity to toxic chemicals. Thus, although PCs may have an embryonic role in cardiogenesis, they do not appear to play a part later in cardiac function as suggested. However, the role of PCs in the uptake and sequestering of toxins, their sensitivity to toxic stress and the decreased lifespan of animals without PCs indicate the importance of PCs in organismal homeostasis.

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Acknowledgements

We are grateful to Richard Cripps, Manfred Frasch, V. Sriram, Pernille Rorth, Helen Skaer and K. VijayRaghavan for antibodies, fly stocks and valuable comments, to B.S. Suma for help with confocal microscopy and to V. K. Sharma for advice on statistical analysis.

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Affiliations

  1. Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur P.O., Bangalore, 560064, India

    Debjani Das, Rajaguru Aradhya, D. Ashoka & Maneesha Inamdar

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  1. Debjani Das
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  2. Rajaguru Aradhya
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  3. D. Ashoka
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  4. Maneesha Inamdar
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Corresponding author

Correspondence to Maneesha Inamdar.

Additional information

D.D. is supported by a CSIR-SPM fellowship. This work was funded by the JNCASR, the Council of Scientific and Industrial Research, and the Department of Biotechnology, Government of India.

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Das, D., Aradhya, R., Ashoka, D. et al. Post-embryonic pericardial cells of Drosophila are required for overcoming toxic stress but not for cardiac function or adult development. Cell Tissue Res 331, 565–570 (2008). https://doi.org/10.1007/s00441-007-0518-z

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  • DOI: https://doi.org/10.1007/s00441-007-0518-z

Keywords

  • Pericardial cell
  • Heartbeat
  • Cardiac remodelling
  • Lifespan
  • Toxic stress
  • Drosophila melanogaster (Insecta)