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Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats

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Abstract

PU.1, which is a transcription factor, promotes the terminal differentiation of alveolar macrophages (AMs). Its expression is regulated by granulocyte/macrophage colony-stimulating factor (GM-CSF). In this study of AMs in newborn rats, we performed immunohistochemical staining, acid phosphatase staining, reverse transcriptase polymerase chain reaction (RT-PCR), quantitative real-time PCR, cytokine assay, and electron microscopy. AMs at 3 and 7 days after birth had a large foamy appearance with an intracytoplasmic accumulation of surfactants. Weak expression of PU.1 was observed in the nuclei. AMs at 15 days after birth were smaller, and PU.1 expression had increased. Ultrastructurally, AMs at 1 day after birth had a smooth surface and abundant lamellar structures in the cytoplasm, whereas AMs at 56 days after birth were characterized by (1) abundant microvillar projections on the cell surface, and (2) well-developed lysosomes and a few lamellar structures in the cytoplasm. Acid phosphatase activity and the expression of mannose receptor, scavenger receptor, and GM-CSF receptor α were enhanced in AMs with time after birth. These results suggest that AMs are initially immature, and that their terminal differentiation starts after birth concomitantly with an increased expression of PU.1.

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Acknowledgements

We thank Mr. Susumu Momozaki, Mr. Kenji Oyachi, Mr. Takashi Aoyama, (Division of Cellular and Molecular Pathology, Niigata University Graduate School of Medical and Dental Science), and Ms. Yoko Aizawa (Bioscience and Medical Research Center, Niigata University Medical and Dental Hospital) for their excellent technical assistance.

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Authors and Affiliations

  1. Division of Cellular and Molecular Pathology, Department of Cellular Function, Niigata University Graduate School of Medical and Dental Sciences, Asahimachi 1-757, Niigata, 951-8510, Japan

    Haruko Iwabuchi, Takashi Kawasaki, Takashi Yamamoto & Makoto Naito

  2. Division of Pediatrics, Department of Homeostatic Regulation and Development, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

    Haruko Iwabuchi & Makoto Uchiyama

  3. Bioscience and Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan

    Koh Nakata

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  1. Haruko Iwabuchi
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  2. Takashi Kawasaki
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  3. Takashi Yamamoto
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  4. Makoto Uchiyama
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  5. Koh Nakata
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  6. Makoto Naito
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Correspondence to Makoto Naito.

Additional information

This study was supported in part by grant no. H14-trans-014 from the Ministry of Health, Labor, and Welfare of Japan and grants from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Iwabuchi, H., Kawasaki, T., Yamamoto, T. et al. Expression of PU.1 and terminal differentiation of alveolar macrophages in newborn rats. Cell Tissue Res 329, 71–79 (2007). https://doi.org/10.1007/s00441-007-0405-7

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