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Postnatal expression and denervation induced up-regulation of aquaporin-5 protein in rat sweat gland

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Abstract

The evolution of aquaporin-5 (AQP5) expression during postnatal development has not been defined in the sweat gland. Previous studies have suggested that AQP isoforms in several peripheral targets are regulated by a neural mechanism. We have examined, in rat sweat glands, the expression of AQP5 during postnatal development and the effects of denervation on AQP5 expression. Both AQP5 mRNA and protein begin to be expressed at postnatal day 10, before sweat-secretory responsiveness first appears; this expression coincides with the occurrence of vasoactive intestinal peptide (VIP) immunoreactivity. Early noradrenergic and later cholinergic interaction between sweat glands and their innervation are disrupted by neonatal chemical sympathectomy or postnatal severance of the sciatic nerve. Examination of such denervated developing rats has shown that secretory responsiveness fails to arise later in the adults, and AQP5 immunostaining increases in the denervated glands, whereas gland morphogenesis and the occurrence of AQP5 expression proceed normally. Immunobloting has revealed an increase of AQP5 abundance after the denervated mature glands lose their secretory ability. These findings suggest that AQP5 protein is necessary for sweat secretion, and that the expression of AQP5 in rat sweat glands is independent of sympathetic innervation. Our data also indicate that factor(s) regulating the normal morphological development of sweat gland might be responsible for controlling AQP5 expression.

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Acknowledgments

We thank Prof. Zhi-Ren Rao, Hong-Ge Jia, and Liang-Wei Chen for morphological expertise and helpful advice, and Li Duan and Rong Cao for skillful technical assistance.

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Authors and Affiliations

  1. Department of Neurology, Xijing Hospital, Fourth Military Medical University, 17 Changle West Road, Xi’an, 710032, People’s Republic of China

    Lei Ma, Yuan-Gui Huang, Yan-Chun Deng, Ji-Yu Tian & Gang Zhao

  2. Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Hua He

  3. Department of Physiology, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Hai-Feng Zhang

  4. Department of Biochemistry and Molecular Biology, Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

    Hong-Lei Che

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  1. Lei Ma
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  2. Yuan-Gui Huang
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  3. Hua He
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  4. Yan-Chun Deng
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  7. Ji-Yu Tian
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  8. Gang Zhao
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Correspondence to Yuan-Gui Huang.

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Ma, L., Huang, YG., He, H. et al. Postnatal expression and denervation induced up-regulation of aquaporin-5 protein in rat sweat gland. Cell Tissue Res 329, 25–33 (2007). https://doi.org/10.1007/s00441-007-0399-1

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  • DOI: https://doi.org/10.1007/s00441-007-0399-1

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