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VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration

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Abstract

Membrane trafficking is one of the most important mechanisms involved in the establishment and maintenance of the forms and functions of the cell. However, it is poorly understood in skeletal muscle cells. In this study, we have focused on vesicle-associated membrane proteins (VAMPs), which are components of the vesicle docking and fusion complex, and have performed immunostaining to investigate the expression of VAMPs in rat skeletal muscle tissue. We have found that VAMP2, but not VAMP1 or VAMP3, is expressed in satellite cells. VAMP2 is also expressed in myofibers in the soleus muscle and nerve endings. This is consistent with previous studies in which VAMP2 has been shown to regulate GLUT4 trafficking in slow-twitch myofibers in soleus muscle and neurotransmitter release in nerve endings. As satellite cells are quiescent myogenic cells, the expression of VAMP2 has further been examined in regenerating muscles after injury by the snake venom, cardiotoxin; we have observed enhanced expression of VAMP2 in immature myotubes with a peak at 3 days after injury. Our findings suggest that VAMP2 plays roles in quiescent satellite cells and is involved in muscle regeneration. The nature of the material transported in the VAMP2-bearing vesicles in satellite cells and myotubes is still under investigation.

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Acknowledgments

We thank Ms. Harumi Matsuda for technical and secretarial assistance, and Ms. Fumiko Ebihara, Mr. Yoshihiro Matsuda, and Ms. Ritsuko Tsukagoshi for technical assistance during a part of the course, Practice in Basic Medical Science, at Gunma University School of Medicine.

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Authors and Affiliations

  1. Department of Neuromuscular and Developmental Anatomy, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Yuki Tajika, Mahito Sato, Tohru Murakami & Hiroshi Yorifuji

  2. Department of Anatomy and Cell Biology, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma, 371-8511, Japan

    Kuniaki Takata

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  1. Yuki Tajika
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  2. Mahito Sato
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  3. Tohru Murakami
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  4. Kuniaki Takata
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  5. Hiroshi Yorifuji
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Correspondence to Hiroshi Yorifuji.

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This work was supported by a research grant (17A-10) for nervous and mental disorders from the Ministry of Health, Labor, and Welfare of Japan, and Grants-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.

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Tajika, Y., Sato, M., Murakami, T. et al. VAMP2 is expressed in muscle satellite cells and up-regulated during muscle regeneration. Cell Tissue Res 328, 573–581 (2007). https://doi.org/10.1007/s00441-006-0376-0

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  • DOI: https://doi.org/10.1007/s00441-006-0376-0

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