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Localisation of caveolin in mammary tissue depends on cell type

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Abstract

Caveolins, components of caveolae, are expressed in mammary tissue. In order to determine whether caveolins are present in different mammary cell types and whether their localisation depends on the physiological stage or species, cav-1 and cav-2 were characterised by immunoblotting in mammary tissues from the mouse, ewe and rabbit and localised, by immunofluorescence and electron microscopy, in mammary tissues from the mouse and ewe. At all the physiological stages studied, cav-1 and cav-2 were present in endothelial and myoepithelial cells in which flask-shaped caveolae were abundant. However, labelling of cav-1 and cav-2 associated with small vesiculo-tubular structures (including those close to lipid droplets) was low in epithelial cells. To study the possible association of cav-1 with lipid droplets, lactating ewe mammary fragments were treated in vitro with brefeldin A. This treatment did not modify the association of cav-1-labelled structures with lipid droplets. Finally, HC11 and MCF-10A mammary cell lines were treated with oleic acid. The total quantity of cav-1 was little affected by the treatment, although the lipid droplet labelling of cav-1 was amplified in MCF-10A cells. Thus, the synthesis and localisation of caveolins are mostly dependent upon the cell types of mammary tissue and upon their state of differentiation.

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Acknowledgments

We are grateful to Drs. Eve Devinoy and Eric Chanat for their helpful comments, to Serge Delpal for his competent assistance and to Marie-Elisabeth Marmillod for her secretarial skills.

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Authors and Affiliations

  1. Unité UR1196 Génomique et Physiologie de la Lactation, INRA, 78352, Jouy-en-Josas cedex, France

    Catherine Hue-Beauvais, Christine Péchoux, Edwige Bouguyon, Sophie Chat, Sandrine Truchet, Alain Pauloin, Yann Le Gouar & Michèle Ollivier-Bousquet

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  1. Catherine Hue-Beauvais
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  2. Christine Péchoux
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  3. Edwige Bouguyon
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  4. Sophie Chat
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  5. Sandrine Truchet
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  6. Alain Pauloin
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  7. Yann Le Gouar
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  8. Michèle Ollivier-Bousquet
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Correspondence to Michèle Ollivier-Bousquet.

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Hue-Beauvais, C., Péchoux, C., Bouguyon, E. et al. Localisation of caveolin in mammary tissue depends on cell type. Cell Tissue Res 328, 521–536 (2007). https://doi.org/10.1007/s00441-006-0370-6

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  • DOI: https://doi.org/10.1007/s00441-006-0370-6

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