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In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses

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Abstract

Non-invasive intratissue ablation was performed in the cornea of living rabbits by using 80 MHz near-infrared intense nanojoule femtosecond laser pulses. The intratissue surgical effect was induced by multiphoton absorption at a wavelength of 800 nm and was ascertained by histological examination. Highly precise intratissue ablation was obtained with no detrimental effects to the overlying or underlying layers. Activated keratocytes in the laser-treated corneas were detected with two-photon imaging postoperatively. Intratissue femtosecond laser ablation thus has potential as a effective technique in refractive surgery for the treatment of visual disorders.

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Acknowledgments

We gratefully acknowledge the technical support from JenLab, which provided the laser and the modified microscope. We thank Prof. Chris P. Lohmann (Eye Hospital Rechts der Isar, TU Munich), Christof Donitzky (WaveLight), Prof. Karl-Otto Greulich (Institute of Molecular Technology), Dr. Allan Spessa (Max Planck Institute), and Shuping Song (University of Jena) for their stimulating discussions. We are also grateful to Sigrun Kirste (Institute of Laboratory Animal Science) and to Ursula Eschler, Isa Lemke, Michael Szabó, and Helmut Hörig (Institute of Anatomy II) for their outstanding technical assistance.

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Authors and Affiliations

  1. Institute of Microscopic Anatomy (Anatomy II), University of Jena, Teichgraben 7, 07743, Jena, Germany

    Bao-Gui Wang & Karl-Juergen Halbhuber

  2. Fraunhofer Institute of Biomedical Technology (IBMT), Ensheimer Strasse 48, 66386, St. Ingbert, Germany

    Iris Riemann & Karsten Koenig

  3. Institute of Laboratory Animals Science, University of Jena, Dornburger Strasse 23, 07743, Jena, Germany

    Harald Schubert

  4. Faculty of Physics and Mechatronics, Saarland University, 66123, Saarbrücken, Germany

    Karsten Koenig

  5. JenLab, Schillerstrasse 1, 07745, Jena, Germany

    Karsten Koenig

Authors
  1. Bao-Gui Wang
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  2. Iris Riemann
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  3. Harald Schubert
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  4. Karl-Juergen Halbhuber
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  5. Karsten Koenig
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Corresponding author

Correspondence to Karsten Koenig.

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This work was supported in part by the German Science Foundation.

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Wang, BG., Riemann, I., Schubert, H. et al. In-vivo intratissue ablation by nanojoule near-infrared femtosecond laser pulses. Cell Tissue Res 328, 515–520 (2007). https://doi.org/10.1007/s00441-006-0367-1

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  • DOI: https://doi.org/10.1007/s00441-006-0367-1

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