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Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in goldfish brain

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Abstract

Intracerebroventricular (ICV) injection of melanin-concentrating hormone (MCH) influences feeding behavior in the goldfish and exerts an anorexigenic action in goldfish brain, unlike its orexigenic action in mammals. Despite a growing body of knowledge concerning MCH function in mammals, the role of MCH in appetite has not yet been well studied in fish. The aim of the present study was to investigate the involvement of endogenous MCH in the feeding behavior of the goldfish. We examined the distribution of MCH-like immunoreactivity (MCH-LI) in the goldfish brain and the effect of feeding status upon this distribution. Neuronal cell bodies containing MCH-LI were localized specifically to four areas of the hypothalamus. Nerve fibers with MCH-LI were found mainly in the neurohypophysis, with a few in the telencephalon, mesencephalon, and diencephalon. The number of neuronal cell bodies containing MCH-LI in the dorsal area adjoining the lateral recess of the third ventricle in the posterior and inferior lobes of the hypothalamus showed a significant decrease in fasted fish compared with that in normally fed fish, although other areas showed no evident differences. We also administered an antiserum against fish MCH (anti-MCH serum) by ICV injection and examined its immunoneutralizing effect on food intake by using an automatic monitoring system. Cumulative food intake was significantly increased by ICV injection of the anti-MCH serum. These results indicate that MCH potentially functions as an anorexigenic neuropeptide in the goldfish brain, and that the further study of the evolutionary background of the MCH system and its role in appetite is warranted.

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Authors and Affiliations

  1. Laboratory of Regulatory Biology, Graduate School of Science and Engineering, University of Toyama, 3190-Gofuku, Toyama, 930-8555, Japan

    Kouhei Matsuda, Sei-Ichi Shimakura, Tohru Miura, Keisuke Maruyama & Minoru Uchiyama

  2. Laboratory of Molecular Endocrinology, School of Fisheries Sciences, Kitasato University, Iwate, 022-0101, Japan

    Hiroshi Kawauchi & Akiyoshi Takahashi

  3. Department of Anatomy, Showa University School of Medicine, Tokyo, 142-8555, Japan

    Seiji Shioda

Authors
  1. Kouhei Matsuda
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  2. Sei-Ichi Shimakura
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  3. Tohru Miura
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  4. Keisuke Maruyama
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  5. Minoru Uchiyama
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  6. Hiroshi Kawauchi
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  7. Seiji Shioda
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  8. Akiyoshi Takahashi
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Corresponding author

Correspondence to Kouhei Matsuda.

Additional information

This work was supported by Grants-in-Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (K.M. and A.T.) and by a research grant from the Toyama Marine Biotechnology Association (K.M.).

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Matsuda, K., Shimakura, SI., Miura, T. et al. Feeding-induced changes of melanin-concentrating hormone (MCH)-like immunoreactivity in goldfish brain. Cell Tissue Res 328, 375–382 (2007). https://doi.org/10.1007/s00441-006-0347-5

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  • DOI: https://doi.org/10.1007/s00441-006-0347-5

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