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Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar

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Abstract

In all vertebrates, the synthesis and release of prolactin (Prl) from pituitary lactotroph cells is tightly controlled by hypothalamic factors. We have cloned and characterized a hypothalamic cDNA from Atlantic salmon (Salmo salar) encoding C-RFa, a peptide structurally related to mammalian Prl-releasing peptide (PrRP). The deduced preprohormone precursor is composed of 155 amino acid residues presenting a 87.1% similarity to chum salmon C-RFa and a 100% similarity to all fish C-RFa in the bioactive precursor motifs. C-RFa-immunoreactive perikarya and fibres were located in the brain of S. salar, especially in the hypothalamus, olfactory tract, optic tectum and cerebellum. In contrast, immunolabelled fibres were not observed in the pituitary stalk or in the hypophysis. However, interestingly, we detected immunolabelled cells in the rostral pars distalis of the pituitary in the basolateral region in which Prl is synthesized. These results were confirmed by obtaining a strong signal by using reverse transcription/polymerase chain reaction (RT-PCR) on mRNA from both hypothalamus and pituitary. These data show, for the first time, by immunohistochemistry and RT-PCR, that C-RFa is produced in pituitary cells. Finally, based on these results, a possible function for C-RFa as a locally produced PrRP in this teleost is discussed.

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Authors and Affiliations

  1. Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile

    R. A. Montefusco-Siegmund, A. Romero, G. Kausel & J. Figueroa

  2. Laboratoire de Biologie Moléculaire et de Génie Génétique, Université de Liége, Institut de Chimie B6, B-4000, Sart-Tilman, Belgium

    M. Muller

  3. Department of Biological Science, Faculty of Life and Environmental Science, Shimane University, Matsue, Japan

    M. Fujimoto

Authors
  1. R. A. Montefusco-Siegmund
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  2. A. Romero
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  3. G. Kausel
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  4. M. Muller
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  5. M. Fujimoto
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  6. J. Figueroa
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Correspondence to J. Figueroa.

Additional information

This work was supported by grant no. 1040073 from FONDECYT, Chile, by grant MECESUP AUS 0006 and by a grant from the French Speaking Community of Belgium, University of Liege and by AGCI, Chile.

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Montefusco-Siegmund, R.A., Romero, A., Kausel, G. et al. Cloning of the prepro C-RFa gene and brain localization of the active peptide in Salmo salar . Cell Tissue Res 325, 277–285 (2006). https://doi.org/10.1007/s00441-006-0168-6

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  • DOI: https://doi.org/10.1007/s00441-006-0168-6

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