Skip to main content

Advertisement

SpringerLink
Log in

Expression of osteocalcin and Jun D in the early period during reactionary dentin formation after tooth preparation in rat molars

  • Regular Article
  • Published:
Cell and Tissue Research Aims and scope Submit manuscript

Abstract

We examined, in rats, the expression of osteocalcin and Jun D in the early stage of reactionary dentin formation after tooth preparation and the accompanying morphological changes. Reverse transcription/polymerase chain reaction analysis revealed strong expression of osteocalcin mRNA in pulp tissue at 2 and 3 days post-preparation compared with that in control teeth. Light microscopy demonstrated that, at the dentin–pulp interface, damaged odontoblasts were detached from the dentin matrix immediately after preparation, with neutrophils lining the dental surface after 1 day. After 2–3 days, differentiated odontoblasts appeared at the interface. Reactionary dentin with tubular structures was formed under the cavity after 10 days. Immunoelectron microscopy showed that trace amounts of osteocalcin were expressed in odontoblasts at 2 days post-preparation, and abundant osteocalcin was found in the highly developed Golgi apparatus and granules at 3 days post-preparation. Osteocalcin was also found on type I collagen fibrils in newly formed predentin. The existing dentinal tubules were filled with osteocalcin-coated type I collagen fibrils. We observed, by immnohistochemistry, that Jun D was temporally expressed in the nuclei of the odontoblasts at 1 and 2 days post-preparation. However, no Jun D was found in the dental pulp cells at any other time or in control teeth. Thus, osteocalcin expression is correlated with reactionary dentin formation, and Jun D is associated with osteocalcin expression in odontoblasts. Osteocalcin may also serve as an obturator of the dentinal tubules to protect dental pulp vitality against external irritants after preparation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Alexandre C, Thornin E, Olga B, Pierre L, Patrick PM (2003) Inflammatory cytokine expression is independent of the c-Jun N-terminal kinase/AP-1 signaling cascade in human neutrophils. J Immunol 171:3751–3761

    Google Scholar 

  • Angel P, Karlin M (1991) The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation. Biochem Biophys Acta 1072:129–157

    Article  CAS  PubMed  Google Scholar 

  • Boivin G, Morel G, Lian JB, Anthoine-Terrier C, Dubois PM, Meunier PJ (1990) Localization of endogenous osteocalcin in neonatal rat bone and its absence in articular cartilage: effect of warfarin treatment. Virchows Archiv A Pathol Anat Histopathol 417:505–512

    Google Scholar 

  • Boskey AL, Gadaleta S, Gundberg C, Doty SB, Ducy P, Kersenty G (1998) Fourier transform infrared microscopic analysis of bones of osteocalcin-deficient mice provides insight into the function of osteocalcin. Bone 23:187–196

    Article  Google Scholar 

  • Camarda AJ, Butleer WT, Finkelman RD, Nanci A (1987) Immunocytochemical localization of gamma-carboxyglutamic acid-containing proteins (osteocalcin) in rat bone and dentin. Calcif Tissue Int 40:349–355

    Google Scholar 

  • Couve E (1986) Ultrastructural changes during the life cycle of human odontoblast. Arch Oral Biol 31:643–651

    Article  Google Scholar 

  • D’Souza RN, Backman T, Baumgardner KR, Butler WT, Litz M (1995) Characterization of cellular responses involved in reparative dentinogenesis in rat molars. J Dent Res 74:702–709

    Google Scholar 

  • Ducy P, Desbois C, Boyce B, Pinero G, Dunstan C, Smith E, Bonadio J, Goldstein S, Gundberg C, Bradley A, Karsenty G (1996) Increased bone formation in osteocalcin-deficient mice. Nature 382:448–452

    Article  CAS  PubMed  Google Scholar 

  • Karsenty G, Wagner EF (2002) Reaching a genetic and molecular understanding of skeletal development. Dev Cell 2:389–406

    Article  CAS  PubMed  Google Scholar 

  • Kitamura C, Terashita M (1997) Expressions of c-jun and jun-B proto-oncogenes in odontoblasts during development of bovine tooth germs. J Dent Res 76:822–830

    Google Scholar 

  • Kitamura C, Kimura K, Nakayama T, Terashita M (1999) Temporal and spatial expression of c-jun and jun-B proto-oncogenes in pulp cells involved with reparative dentinogenesis after cavity preparation of rat molars. J Dent Res 78:673–680

    Google Scholar 

  • Linde A, Bhown M, Cothran WC, Hoglund A, Butler WT (1982) Evidence for several gamma-carboxyglutamic acid containing proteins in dentin. Biochim Biophys Acta 704:235–239

    Google Scholar 

  • McCabe LR, Stein JL, Lian JB, Bravo R, Stein GS (1995) Selective expression of fos and jun related genes during osteoblast proliferation and differentiation. Exp Cell Res 217:255–262

    Article  Google Scholar 

  • McCabe LR, Banerjee C, Kundu R, Harrison J, Dobner PR, Stein JL, Lian JB, Stein GS (1996) Developmental expression and activities of specific Fos and Jun proteins are functionally related to osteoblast maturation: role of Fra-2 and Jun D during differentiation. Endocrinology 137:4398–4408

    Article  CAS  PubMed  Google Scholar 

  • Mckee MD, Farach-Carsn MC, Butler WT, Hauschka PV, Nanci A (1993) Ultrastructural immunolocalization of noncollagenous (osteopontin and osteocalcin) and plasma (albumin and alpha 2HS-glycoprotein) proteins in rat bone. J Bone Miner Res 8:485–496

    CAS  PubMed  Google Scholar 

  • Mckee MD, Zalzal S, Nanci A (1996) Extracellular matrix in tooth cementum and mantle dentin: localization of osteopontin and other noncollagenous proteins, plasma proteins, and glycoconjugates by electron microscopy. Anat Rec 245:293–312

    Article  CAS  PubMed  Google Scholar 

  • Mosavin R, Mellon WS (1996) Posttranscriptional regulation of osteocalcin mRMA in clonal osteoblast cells by 1,25-dehydroxyvitamin D3. Arch Biochem Biophys 332:142–152

    Article  Google Scholar 

  • Nakashima M, Nagasawa H, Yamada Y, Reddi AH (1994) Regulatory role of transforming growth factor-beta, bone morphogenetic protein-2, and protein-4 on gene expression of extracellular matrix proteins and differentiation of dental pulp cells. Dev Biol 162:18–28

    Article  Google Scholar 

  • Owen TA, Aronow M, Shalhoub V, Barone LM, Wilming L, Tassinari MS, Kennedy MB, Pockwinse S, Lian JB, Stein GS (1990a) Progressive development of the rat osteoblast phenotype in vitro: reciprocal relationships in expression of genes associated with osteoblast proliferation and differentiation during formation of the bone extracellular matrix. J Cell Physiol 143:420–430

    Google Scholar 

  • Owen TA, Bortell R, Yocum SA, Smock SL, Zang M, Abate C (1990b) Coordinate occupancy of AP-1 sites in the vitamin D-responsive and CCAAT box elements by Fos-Jun in the osteocalcin gene: model for phenotype suppression of transcription. Proc Natl Acad Sci USA 87:9990–9994

    Google Scholar 

  • Pashley DH, Nelson R, Pashley EL (1981) In-vivo fluid movement across dentine in the dog. Arch Oral Biol 26:707–710

    Article  Google Scholar 

  • Seltzer S, Bender IB (1984) Mechanical and thermal irritants. In: Seltzer S, Bender IB (eds) The dental pulp, 3rd edn. Lippencott, Philadelphia, pp 195–214

    Google Scholar 

  • Smith AJ, Cassidy N, Perry H, Begue-Kirn C, Ruch JV, Lesot H (1995) Reactionary dentinogenesis. Int J Dev Biol 39:273–280

    Google Scholar 

  • Stein GS, Lian JB, Owen TA (1990) Relationship of cell growth to the regulation of tissue-specific gene expression during osteoblast differentiation. FASEB J 4:3111–3123

    Google Scholar 

  • TenCate AR (1998) Hard tissue formation and destruction. In: TenCate AR (ed) Oral histology, 5th edn. Mosby, St. Louis, pp 69–78

    Google Scholar 

  • Torneck CD (1998) Dentin-pulp complex. In: TenCate AR (ed) Oral histology, 5th edn. Mosby, St. Louis, pp 150–196

    Google Scholar 

  • Tziafas D (1994) Mechanisms controlling secondary initiation of dentinogenesis: a review. Int Endod J 27:61–74

    Google Scholar 

  • Yamaza T, Kido MA, Kiyoshima T, Nishimura Y, Himeno M, Tanaka T (1997) A fluid-phase endocytotic capacity and intracellular degradation of a foreign protein (horseradish peroxidase) by lysosomal cysteine proteinases in the rat junctional epithelium. J Periodont Res 32:651–660

    Google Scholar 

  • Yoon K, Rutledge SJC, Buenaga RF, Rodan GA (1988) Characterization of the rat osteocalcin gene: stimulation of promotor activity by 1,25-dihydroxyvitamin D3. Biochemistry 27:8521–8526

    Google Scholar 

Download references

Acknowledgements

We are grateful to Prof. Teruo Tanaka (Kyushu University Graduate School of Dental Science) for helpful suggestions and Dr. Teiichi Ibuki (General Oral Care Unit, Kyushu University Hospital) for valuable technical support.

Author information

Authors and Affiliations

  1. Departments of Endodontology and Operative Dentistry, Kyushu University Graduate School of Dental Science, Fukuoka, 812-8582, Japan

    Masako Hirata, Yu Feng Mei & Akifumi Akamine

  2. Departments of Oral Anatomy and Cell Biology, Kyushu University Graduate School of Dental Science, Fukuoka, 812-8582, Japan

    Takayoshi Yamaza & Yu Feng Mei

Authors
  1. Masako Hirata
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takayoshi Yamaza
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu Feng Mei
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Akifumi Akamine
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masako Hirata.

Additional information

This work was supported by the Ministry of Education, Science, Sports, and Culture of Japan (Grants-in-Aid for Scientific Research (C) no. 09671958 and no. 12671855 to M. Hirata).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hirata, M., Yamaza, T., Mei, Y.F. et al. Expression of osteocalcin and Jun D in the early period during reactionary dentin formation after tooth preparation in rat molars. Cell Tissue Res 319, 455–465 (2005). https://doi.org/10.1007/s00441-004-1035-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00441-004-1035-y

Keywords

Search

Navigation