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mRNA for pancreatic uncoupling protein 2 increases in two models of acute experimental pancreatitis in rats and mice

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Abstract

Uncoupling-protein 2 (UCP2) is a mitochondrial protein that appears to be involved in cellular oxidant defense and in the regulation of oncotic cell death, both of which are important features of acute pancreatitis. However, UCP2 expression in acute pancreatitis has not been previously reported. In the current experiments, pancreatic gene expression was studied by real-time reverse-transcription/polymerase chain reaction and Northern blots. Two models of acute experimental pancreatitis were investigated: cerulein-induced pancreatitis in mice at two different time points and taurocholate-induced pancreatitis in rats at two degrees of severity. After cerulein administration, acinar injury and leukocyte infiltration was significantly higher at 24 h compared with 12 h after the first injection of cerulein (P<0.05, P<0.005, respectively). UCP2 mRNA was unchanged at 12 h but was nearly 12-fold greater than control levels after 24 h (P<0.001). UCP2 gene expression correlated with acinar injury (r=0.69; P<0.001). By 72 h after taurocholate administration, the severe group had more necrosis than the mild group (P<0.005). Pancreatic UCP2 mRNA was increased fourfold in the severe group compared with controls (P<0.01). UCP2 expression correlated with parenchymal necrosis (r=0.61; P<0.01). Thus, pancreatic UCP2 mRNA increased in two models of acute pancreatitis. The increase in UCP2 gene expression was correlated with the severity of the disease. Up-regulation of UCP2 in the pancreas may be a protective response to oxidative stress, but this increase may also have a negative influence on cellular energy metabolism. Therefore, acinar UCP2 may be an important modifier of the severity of acute pancreatitis.

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Acknowledgements

We thank Irene Engström, Beth Hagman, Ulla Gülich, and Maria Sylvàn for their skillful assistance.

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Authors and Affiliations

  1. Division of Surgery, Center for Surgical Sciences, K53, Karolinska Institutet, Karolinska University Hospital Huddinge, 141 86, Stockholm, Sweden

    Ralf Segersvärd, Bengt Isaksson & Johan Permert

  2. Department of Cell and Molecular Biology, Biomedical Center, B1122b, 221 84, Lund, Sweden

    Catarina Rippe, Marie DuPlantier & Charlotte Erlanson-Albertsson

  3. Department of Biology, Adams State College, Alamosa, CO 81102, USA

    Margery K. Herrington

  4. Department of Surgery, Feinberg School of Medicine, Northwestern University, 303 East Chicago Avenue, Tarry 4-711, Chicago, IL 60611, USA

    Thomas E. Adrian

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  1. Ralf Segersvärd
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  2. Catarina Rippe
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  4. Margery K. Herrington
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  5. Bengt Isaksson
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  7. Charlotte Erlanson-Albertsson
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Corresponding author

Correspondence to Ralf Segersvärd.

Additional information

This study was made possible by collaboration within the Pancreas 2000 project and was supported by grants from the Swedish Medical Research Council (K2003-73X-12665-06A and K2003-03X-07904-17C) and the Swedish Cancer Society (4572-B02-02XBB).

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Segersvärd, R., Rippe, C., DuPlantier, M. et al. mRNA for pancreatic uncoupling protein 2 increases in two models of acute experimental pancreatitis in rats and mice. Cell Tissue Res 320, 251–258 (2005). https://doi.org/10.1007/s00441-004-1024-1

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