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Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars

Cell and Tissue Research volume 317, pages 299–312 (2004)Cite this article

Abstract

To elucidate the roles of proteoglycans (PGs), bone sialoprotein (BSP), and osteopontin (OPN) in cementogenesis, their distribution was investigated in developing and established acellular cementum of rat molars by an immunoperoxidase method. To characterize PGs, antibodies against five species of glycosaminoglycans (GAGs), chondroitin-4-sulfate (C4S), chondroitin-6-sulfate (C6S), unsulfated chondroitin (C0S), dermatan sulfate (DS), and keratan sulfate (KS) were used. Routine histological staining was also applied. With onset of dentin mineralization, the initial cementum appeared on the dentin surface as a hematoxylin-stained fibril-poor layer. Subsequently, primitive principal fibers attached to the initial cementum. As the acellular cementum containing extrinsic fibers covered the initial cementum, the initial cementum formed the cemento-dentinal junction. Following immunohistochemistry at the earliest time of cementogenesis, the initial cementum was intensely immunoreactive for C4S, C6S, C0S, BSP, and OPN. After the initial cementum was embedded, neither the cemento-dentinal junction nor the cementum was immunoreactive for any GAG species. However, the cementum and cemento-dentinal junction were consistently immunoreactive for BSP. Although the cemento-dentinal junction was consistently immunoreactive for OPN, the remaining cementum showed no significant immunoreactivity. Thus, initial acellular cementogenesis requires a dense accumulation of PGs, BSP, and OPN, which may be associated with the mineralization process independently of collagen fibrils and initial principal fiber attachment.

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  1. Department of Oral Health Science, Hokkaido University Graduate School of Dental Medicine, Kita13 Nishi7 Kita-Ku, Sapporo, 060-8586, Japan

    T. Yamamoto, T. Domon, S. Takahashi, A. K. S. Arambawatta & M. Wakita

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  1. T. Yamamoto
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  2. T. Domon
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  3. S. Takahashi
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Yamamoto, T., Domon, T., Takahashi, S. et al. Immunolocalization of proteoglycans and bone-related noncollagenous glycoproteins in developing acellular cementum of rat molars. Cell Tissue Res 317, 299–312 (2004). https://doi.org/10.1007/s00441-004-0896-4

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Keywords

  • Acellular cementum
  • Cemento-dentinal junction
  • Glycosaminoglycans
  • Noncollagenous glycoproteins
  • Rat (Wistar, male)