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Regulation of heat shock protein 47 and type I procollagen expression in avian tendon cells

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Abstract.

Heat shock protein 47 (Hsp47) is a collagen-binding stress protein that acts as a collagen-specific molecular chaperone during the biosynthesis and secretion of procollagen. Type I collagen is a major component of tendons. Coexpression of genes for both proteins has been reported in various tissues, where many growth factors likely regulate their expressions in different ways. Here we describe the effects of increased temperature, mechanical stress and growth factors on Hsp47 and type I procollagen expression in embryonic chicken tendon cells. The expression of Hsp47 mRNA at 45°C increased within 60 min and returned to baseline in 4 h after the temperature decreased to 37°C. Our data also show that transforming growth factor β1 (TGF-β1) is another regulator of Hsp47 expression as the addition of TGF-β1 led to a moderate increase in the expression of Hsp47 mRNA. TGF-β2 and TGF-β3 exerted only a small effect; epidermal growth factor and tumor necrosis factor α (TNF-α) had none. TGF-β1 increased type I procollagen mRNA expression and TNF-α reduced this expression. TGF-β1 delayed the degradation of Hsp47 mRNA after heat shock likely via post-transcriptional regulation of the Hsp47 gene. We also report that mechanical stress increased Hsp47 mRNA expression and Hsp47 protein synthesis. Induction of Hsp47 protein expression by heat shock, mechanical stress and TGF-β1 was likely achieved through activation and translocation of heat shock transcription factor 1 into the nucleus. Our data indicate that TGF-β1 is a major regulator of both procollagen and Hsp47 genes.

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Acknowledgements.

We would like to thank Dr. Kendall Frazier for helpful discussions.

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  1. The Soft Tissue Center, Department of Pathology, College of Veterinary Medicine, The University of Georgia, Athens, GA 30602, USA

    Hongjie Pan & Jaroslava Halper

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  1. Hongjie Pan
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  2. Jaroslava Halper
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Correspondence to Jaroslava Halper.

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This work was supported by a grant from the University of Georgia Research Foundation

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Pan, H., Halper, J. Regulation of heat shock protein 47 and type I procollagen expression in avian tendon cells. Cell Tissue Res 311, 373–382 (2003). https://doi.org/10.1007/s00441-003-0699-z

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  • DOI: https://doi.org/10.1007/s00441-003-0699-z

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