Association of CXCR6 with COVID-19 severity: delineating the host genetic factors in transcriptomic regulation

Abstract

The coronavirus disease 2019 (COVID-19) is an infectious disease that mainly affects the host respiratory system with ~ 80% asymptomatic or mild cases and ~ 5% severe cases. Recent genome-wide association studies (GWAS) have identified several genetic loci associated with the severe COVID-19 symptoms. Delineating the genetic variants and genes is important for better understanding its biological mechanisms. We implemented integrative approaches, including transcriptome-wide association studies (TWAS), colocalization analysis, and functional element prediction analysis, to interpret the genetic risks using two independent GWAS datasets in lung and immune cells. To understand the context-specific molecular alteration, we further performed deep learning-based single-cell transcriptomic analyses on a bronchoalveolar lavage fluid (BALF) dataset from moderate and severe COVID-19 patients. We discovered and replicated the genetically regulated expression of CXCR6 and CCR9 genes. These two genes have a protective effect on lung, and a risk effect on whole blood, respectively. The colocalization analysis of GWAS and cis-expression quantitative trait loci highlighted the regulatory effect on CXCR6 expression in lung and immune cells. In the lung-resident memory CD8+ T (TRM) cells, we found a 2.24-fold decrease of cell proportion among CD8+ T cells and lower expression of CXCR6 in the severe patients than moderate patients. Pro-inflammatory transcriptional programs were highlighted in the TRM cellular trajectory from moderate to severe patients. CXCR6 from the 3p21.31 locus is associated with severe COVID-19. CXCR6 tends to have a lower expression in lung TRM cells of severe patients, which aligns with the protective effect of CXCR6 from TWAS analysis.

Introduction

The coronavirus disease 2019 (COVID-19) pandemic has already infected over 1.6 million people and caused numerous morbidities and over 3.4 million death worldwide as of May 2021. The virus is evolving fast with new variants being emerged in the world (Liu et al. 2020a; Zhao et al. 2004). A huge disparity in the severity of symptoms in different patients has been observed. In some of the patients, only mild symptoms or even no symptoms are shown, and little treatment or interventions are required, while a subset of patients experience rapid disease progression to respiratory failure and need urgent and intensive care (Wu and McGoogan 2020). Although age and sex are major risk factors of COVID-19 disease severity (Bhopal and Bhopal 2020), it remains largely unclear about the factors leading to the variability on COVID-19 severity and which group of individuals confer intrinsic susceptibility to COVID-19.

Several genome-wide association studies (GWAS) have been carried out, and one genomic risk locus, 3p21.31, has been replicated to be associated with the critical illness. One recent study by the Severe COVID-19 GWAS Group identified 3p21.31 risk locus for the susceptibility to severe COVID-19 with respiratory failure (Severe Covid et al. 2020). This GWAS signal was then replicated in a separate meta-analysis comprising total 2972 cases from nine cohorts by COVID-19 Host Genetics Initiative (HGI) round 4 alpha. However, there is a cluster of six genes (SLC6A20, LZTFL1, CCR9, FYCO1, CXCR6, and XCR1) nearby the lead SNP rs35081325 within a complex linkage disequilibrium (LD) structure, which makes the “causal” gene and functional implication of this locus remain elusive (Pairo-Castineira et al. 2020; Severe Covid et al. 2020).

The majority of GWAS variants are located in non-coding loci, many of which are in the enhancer or promoter regions, playing roles as cis- or trans-regulatory elements to alter gene expression (GTEx Consortium 2020). Although the function of non-coding variants could not be directly interrupted by their locations, their mediation effect on gene expression could be inferred by the expression quantitative trait loci (eQTL) analysis. In recent years, large consortia like GTEx (Genotype-Tissue Expression), eQTLGen Consortium, and DICE (database of immune cell expression) have generated rich eQTLs resources in diverse tissues and immune-related cell types (GTEx Consortium 2020; Schmiedel et al. 2018; Võsa et al. 2018a). A variety of statistical approaches, such as transcriptome-wide association study (TWAS) analysis and colocalization analysis, have successfully interpreted the target genes of non-coding variants by integrating the context-specific eQTLs (Dai et al. 2020; Dai et al. 2019; Gamazon et al. 2015; Giambartolomei et al. 2014).

Recent advances in single-cell transcriptome sequencing provide unprecedented opportunities to understand the biological mechanism underlying disease pathogenesis at the single-cell and cell type levels (Dai et al. 2021; Mathys et al. 2019; Papalexi and Satija 2018). The recent generation of single-cell RNA sequencing (scRNA-seq) data from the bronchoalveolar lavage fluid (BALF) of moderate and severe COVID-19 patients has revealed the landscape of the gene expression changes in major immune cells. However, the transcriptome alteration in specific subpopulations remains mostly unexplored (Liao et al. 2020).

In this study, we aimed to connect the genetic factors with the context-specific molecular phenotype in COVID-19 patients. As illustrated in Fig. 1, we designed a multi-level workflow to dissect the genetically regulated expression (GReX) that contributed to severe COVID-19. We performed TWAS and colocalization analyses with a broad collection of eQTL datasets at the tissue and cellular levels. We further integrated the BALF single-cell transcriptome dataset to explore the cellular transcriptome alterations in severe and moderate COVID-19 patients. Lastly, we proposed a hypothetical mechanism, connecting our multi-layer evidence in host genetic factors, risk genes, and single-cell transcriptome features with the severity of COVID-19.

Fig. 1
figure1

Workflow of a data-driven study: from genetic factor to molecular phenotype. The study has four major levels. Level 1: We collected the current largest COVID-19 genome-wide association study (GWAS) datasets and a non-duplicated replicate of the severe COVID-19 GWAS dataset. Level 2: We utilized the cutting-edge statistical approaches (transcriptome-wide association study and colocalization analysis) and public functional genomics annotations to dissect the genetic effects on gene expression (“Materials and methods”). Then, we cross-validated our findings of these methods to ensure robustness. Level 3: We adapted single-cell RNA sequencing dataset from COVID-19 bronchoalveolar lavage fluid samples. We applied differentially expressed gene analysis and machine learning methods to characterize the molecular changes of candidate genes at single-cell level from COVID-19 moderate and severe patients. We conducted an extensive literature review to explain our observations. Level 4: We proposed a mechanism for explaining the “causal” association of genetic factors and the severity of COVID-19 patients

Materials and methods

GWAS dataset

We obtained GWAS summary statistics for the phenotype “severe COVID-19 patients vs population” (severe COVID-19) from two separate meta-analyses carried out by the COVID-19 Host Genetics Initiative (HGI, https://www.covid19hg.org/) and the Severe COVID-19 GWAS Group (SCGG) (Severe Covid et al. 2020). The GWASHGI A2 round 4 (alpha) cohort consists of 12,816,037 SNPs from the association study of 2972 very severe respiratory confirmed COVID-19 cases and 284,472 controls with unknown SARS-CoV-2 infection status from nine independent studies in a majority of the European Ancestry population. The GWASSCGG dataset is from the first GWAS of severe COVID-19 (Severe Covid et al. 2020), including 8,431,427 SNPs from the association study conducted from 1980 COVID-19 confirmed patients with severe disease status and 2205 control participants from two separate cohorts in Europe.

Transcriptome-wide association analysis

We performed TWAS analyses of severe COVID-19 using S-PrediXcan (Barbeira et al. 2018) to prioritize GWAS findings and identify eQTL-linked genes. S-PrediXcan is a systematic approach that integrates GWAS summary statistics with publicly available eQTL data to translate the evidence of association with a phenotype from the SNP level to the gene level. Briefly, prediction models were built by a flexible and generic approach multivariate adaptive shrinkage in R package (MASHR) using variants with a high probability of being causal for QTL and tissue expression profiles from the GTEx version 8 (GTEx Consortium 2020; Urbut et al. 2019). We chose three tissues that were relevant to SARS-CoV-2 infection, including lung, whole blood, and spleen. Then, we ran S-PrediXcan scripts (downloaded from https://github.com/hakyimlab/MetaXcan, accessed on 10/10/2020) with each of the three tissue-specific models in two severe COVID-19 GWAS datasets, respectively. The threshold used in TWAS significance was adjusted by Bonferroni multiple-test correction with the ~ 10,000 genes. We defined the strict significance as p < 5 × 10–6 (|z|> 4.56) and suggestive significance as p < 5 × 10–5 (|z|> 4.06).

Colocalization analysis

Colocalization was performed to validate significant TWAS associations using two recent and cutting-edge statistical approaches: eCAVIAR (Hormozdiari et al. 2016) and fastENLOC (Wen et al. 2017), which aim to identify a single genetic variant that has shared causality between expression and GWAS trait. Both eCAVIAR and fastENLOC could assess the colocalization posterior probability (CLPP) for two traits at a locus, while eCAVIAR allows for multiple causal variants and fastENLOC features accountability for allelic heterogeneity in expression traits and high sensitivity of the methodology. We ran eCAVIAR between significant TWAS genes and GWAS trait with a maximum of five causal variants per locus and defined a locus as 50 SNPs up- and down-stream of the tested causal variant, following the recommendation in the original paper. The eCAVIAR was downloaded from https://github.com/fhormoz/caviar/ (accessed on 10/25/2020). The biallelic variants from the 1000 Genomes Project phase III in European ancestry were used as an LD reference (Genomes Project et al. 2015). We defined CLPP > 0.5 as having strong colocalization evidence.

To run fastENLOC, we first prepared probabilistic eQTL annotations to generate the cis-eQTL’s posterior inclusion probability (PIP). Specifically, we applied the tissue-specific data from GTEx and T follicular cell-specific data from the DICE database (Schmiedel et al. 2018) using the integrative genetic association analysis with the deterministic approximation of posteriors (DAP-G) package (Lee et al. 2018). Then, GWAS summary statistics were split into approximately LD-independent regions defined by reference panel from European ancestry, and z-scores were converted to PIP. We downloaded the fastENLOC from https://github.com/xqwen/fastenloc (accessed on 10/25/2020) and followed the guideline to yield regional colocalization probability (RCP) for each independent GWAS locus using each tissue- or cell type-specific eQTL annotation. We defined RCP > 0.5 as having strong colocalization evidence.

Functional genomics annotations

To better understand the potential function of the variants identified by GWAS analyses and how they mediate the regulatory effect, we annotated significant SNPs using publicly available data. We obtained the tissue and cellular level eQTL data from the following resources: 1) the eQTLGen consortium (Võsa et al. 2018b) eQTLs generated from 30,912 whole blood samples; 2) Biobank-based Integrative Omics Studies (BIOS) eQTLs generated from 2116 healthy adults (Zhernakova et al. 2017); 3) The GTEx v8 (GTEx Consortium 2020) eQTLs of the lung, whole blood, and spleen tissues; 4) DICE database (Schmiedel et al. 2018) with cellular eQTLs of nine available T cell subpopulations. To identify the genomic annotation of the significant SNPs, we downloaded the multivariate hidden Markov model (ChromHMM) (Ernst and Kellis 2012) processed chromatin-state data of 17 lung and T cell lines from the Roadmap Epigenomics project (Roadmap Epigenomics et al. 2015). To explore the potential chromatin looping of GWAS locus, we used publicly available chromatin interaction (Hi-C) data (Dixon et al. 2012) at a resolution of 40 Kb on IMR-90, a normal lung fibroblast cell line. The Hi-C data have been used to identify specific baits and targets from distant chromatin regions that frequently interact with each other. Variants within the regulatory regions can be connected to the potential gene targets and thus mediate the gene expression. Statistical tests of bait-target pairs were conducted to define significant bait interaction regions and their targets. The eQTL associations and chromatin-state information and Hi-C interactions were processed and plotted using the R Bioconductor package gviz in R version 4.0.3 (Hahne and Ivanek 2016).

Resident memory CD8+ T cells selection and differentially expressed gene analysis

We use the recently published scRNA-seq dataset of bronchoalveolar lavage fluids (BALF) samples from eight patients (three moderate and five severe) with COVID-19 (Liao et al. 2020; Liu et al. 2020b). We adapted the original annotation (Liao et al. 2020) and followed their method to calculate the resident memory CD8+ T (TRM) cells signature score using 31 markers (14 positive markers and 17 negative markers) for all annotated 2677 CD8+ T cells in moderate and severe samples (Kumar et al. 2017; Pont et al. 2019). We defined the CD8+ T cells with top 50% scores as TRM-like cells. We normalized these 1339 cells and conducted a principal component analysis (PCA) to filter out the outliers assuming that samples in the first two principal components follow a multivariate normal distribution, leaving 1282 cells within 95% confidence interval (Supplementary Figure S1). We specifically checked the cell surface marker of CD8+ T cells. We only kept cells with either CD8A or CD8B expression. To distinguish from the CD4+ TRM, we excluded the cells (9 cells) with CD4 expression. Overall, we kept 1, 101 CD8+ TRM cells (765 from moderate patients and 336 from severe patients). Lastly, we conducted a non-parametric Wilcoxon rank-sum test by the function of “FindAllMarkers” from R package Seurat (Stuart et al. 2019) (version 3.1.5 in R version 3.5.2) to perform the differentially expressed genes (DEG) analysis between moderate and severe patients.

Cellular trajectory and transcriptional program analysis in TRM cells

We used the R package Slingshot (Street et al. 2018) to infer cell transition and pseudotime from the scRNA-seq data. Specifically, we first used the expression data to generate the minimum spanning tree of cells in a reduced-dimensionality space [t-Distributed Stochastic Neighbor Embedding (tSNE) project from top 30 principal components of top 3000 variable genes] assuming there are two major clusters (moderate and severe TRM cells). We then applied the principal curve algorithm (Hastie and Stuetzle 1989) to infer an one-dimensional variable (pseudotime) representing each cellular trajectory along with the transcriptional progression. We used our in-house machine learning tool, DrivAER (Driving transcriptional programs based on AutoEncoder derived relevance scores) (Simon et al. 2020), to identify potential transcriptional programs [e.g., gene sets of pathways or transcription factors (TFs)] that potentially regulate the inferred cellular trajectory between the moderate and severe patients. To avoid the potential noise from the low expression genes, we excluded those genes expressed in < 10% cells. DrivAER took gene expression and pseudotime inferred from previous cellular trajectory results (Slingshot) and calculated each gene’s relevance score by performing cellular manifold using Deep Count AutoEncoder (Eraslan et al. 2019) and a random forest model with out-of-bag score calculation as the relevance score. The transcriptional program annotations were from the hallmark pathway gene sets from MSigDB (Liberzon et al. 2015) and transcription factor (TF) target gene sets from TRRUST (Han et al. 2018). To calculate the relevance score, we used the “calc_relevance” function with the following parameters: min_targets = 10, ae_type = “nb-conddisp”, epoch = 100, early_stop = 3, and hidden_size = “(8,2,8)”. The relevance score (R2 coefficient of determination) indicates the proportion of variance in the pseudotime explained by target genes of transcription factors or genes in the hallmark pathways.

DNA motif recognition analysis of genome-wide significant SNPs

We used the function “variation-scan” of the online tool RSAT (http://rsat.sb-roscoff.fr/index.php, accessed on 01/15/2020) (Nguyen et al. 2018) to predict the binding effect of all the significant SNPs in the 3p21.31 locus. We defined the TF with Bonferroni-corrected p < 0.05 as the significant TF. Later, we compared them with the TF with high relevance score from the DrivAER analysis above. The position weight matrices (PWMs) for all the TFs were downloaded from cis-BP Database (http://cisbp.ccbr.utoronto.ca/) version 2019-06_v2.00) (Weirauch et al. 2014) and sequence logos representing motif-binding sites were generated using R package seqLogo version 1.54.3 in R version 3.5.2.

Results

TWAS analysis identified and replicated two chemokine receptor genes

We utilized the latest S-PrediXcan MASHR models trained with GTEx v8 data for TWAS analyses in lung and whole blood on two GWAS datasets of susceptibility to severe COVID-19 (Urbut et al. 2019). In the HGI cohort, we found that a decreased expression of CXCR6, which encodes C–X–C chemokine receptor type 6, in the lung was associated with an increased risk for the development of severe COVID-19 symptoms (p = 1.57 × 10–17, z =  − 8.53), and this result was then replicated in the SCGG cohort (p = 2.84 × 10–5, z =  − 4.19, suggestive significant) (Fig. 2 and Table 1). Likewise, an increased expression of CCR9, which encodes C–C chemokine receptor type 9, in whole blood was associated with an increased risk for the development of severe COVID-19 complications in GWASHGI cohort (p = 7.90 × 10–11, z = 6.50) and this result was replicated in the other GWASSCGG cohort, (p = 3.78 × 10–10, z = 6.26) (Fig. 2 and Table 1). Whole blood and lung transcriptome models also identified two additional significant TWAS genes that are specific to one of the two cohorts. Increased expression of ABO gene in the lung was associated with risk for the development of severe COVID-19 symptoms in GWASSCGG data set (p = 5.98 × 10–7, z = 4.99). Similarly, increased expression of GAS7 gene (Growth Arrest-Specific 7) in whole blood was associated with an increased risk for the development of COVID-19 symptoms in the GWASHGI data set (p = 8.46 × 10–7, z = 4.92). Lastly in spleen, we identified lower expression of C–C Motif Chemokine Receptor 3 (CCR3) was associated with the severe COVID-19 symptoms (Supplementary Table S1) in GWASHGI as previous TWAS mentioned (Pairo-Castineira et al. 2020). However, we failed to replicate the CCR3 signal in GWASSCGG. Overall, CXCR6 and CCR9 were found and replicated to be associated with COVID-19.

Fig. 2
figure2

Manhattan plots illustrating the z-scores of transcriptome-wide association study (TWAS) genes. TWAS z-scores for two genome-wide association study (GWAS) datasets of susceptibility to severe COVID-19 using lung and whole blood tissue models. The upper panel shows the results from GWASHGI and the lower panel from GWASSCGG (see “Materials and methods”). The round and triangle points denote lung and whole blood tissues, respectively, in the TWAS analysis. Dashed horizontal lines denote the Bonferroni-corrected significance threshold (|z|= 4.56, p < 5 × 10–6). Significant genes were highlighted with their gene symbol

Table 1 Summary of TWAS and colocalization analyses in tissues and cell line

Colocalization analysis validated the mediation effect of CXCR6 between GWAS 3p21.31 locus and severe COVID-19

The TWAS findings might be driven by pleiotropy or linkage effect by the LD structure in the GWAS loci instead of the true mediation effect (Wainberg et al. 2019) (Fig. 3A). To rule out the linkage effect and find further evidence of true colocalization of causal signals in the variants that were significant in both GWAS and eQTL analyses, we performed colocalization analyses on all the genes in the 3p21.31 region including CCR1, CCR2, CCR3, CCRL2, CCR5, XCR1, CCR9, CXCR6, FYCO1, LZTFL1 by eCAVIAR and fastENLOC using several tissue- and cell-line- specific eQTL datasets. eCAVIAR with the eQTL data in lung tissue revealed that the severe COVID-19 association could be mediated by the variants that were associated with the expression of CXCR6 (CLPP = 0.79) (Table 1). And the colocalized SNP rs34068335 (GWASHGI p = 5.02 × 10–22) is also related to the increased monocyte percentage of white cells in a blood-trait GWAS study using PhenoScanner (Kamat et al. 2019). CCR3 in spleen shows colocalization with CLPP = 0.580 and CCR1 in T follicular helper cell shows marginal colocalization with CLPP = 0.10 (Supplementary Table S2). The fastENLOC analysis showed a high RCP between the expression of CXCR6 in T follicular helper cells and GWAS signal in both the GWASHGI cohort (RCP = 0.99) and the GWASSCGG cohort (RCP = 0.99) (Table 1). CCRL2 shows colocalization with RCP = 0.5 in T follicular helper cells in both cohorts. CCR5 shows colocalization with RCP = 0.88 in monocytes in SCGG cohort and RC p = 0.49 in monocytes in HGI cohort (Supplementary Table S3). However, colocalization analysis of CCR9 or other genes did not suggest strong colocalization evidence (CLPP < 0.1 and RCP < 0.1).

Fig. 3
figure3

Functional genomic annotation in the 3p21.31 locus with signals from GWASHGI. a LocusZoom view of the association signals of SNPs in the 3p21.31 locus of GWASHGI. The x-axis is the chromosome position in million base pairs (Mb) on GRCh37 reference genome and y-axis represents the –log10 (p value) from GWASHGI dataset. The color indicates the strength of linkage disequilibrium from the lead SNP rs35081325. The genes within the region are annotated in the lower panel. A vertical blue line labels the position of the lead SNP rs35081325 to denote the relationship of GWAS variants to other datasets: expression quantitative trait (eQTL) (b), chromatin interaction (c), and imputed Roadmap functional elements (d). b The significant eQTLs associated with CXCR6 expression in this region. The cis- eQTL datasets include two whole blood datasets [Biobank-based Integrative Omics Studies (BIOS) QTL and eQTLGen] and one T follicular helper cell dataset (DICE). The y-axis represents the –log10 (p value) from the eQTL studies. c The significant Hi-C interactions in normal lung fibroblast cell line (IMR-90). Blue blocks denote the target and bait regions, and red arcs indicate the interactions between functional elements. d The region annotated with the chromatin-state segmentation track (ChromHMM) from the Roadmap Epigenomics data for T cell and lung tissue. The Roadmap Epigenomics cell line IDs are shown on the left side: E017 (IMR-90 fetal lung fibroblasts Cell Line), E033 (Primary T Cells from cord blood), E034 (Primary T Cells from blood), E038 (Primary T help naïve cells from peripheral blood), E039 (Primary T helper naïve cells from peripheral blood), E040 (Primary T helper memory cells from peripheral blood), E041 (Primary T helper cells PMA-Ionomycin stimulated), E042 (Primary T helper 17 cells PMA-Ionomycin stimulated), E043 (Primary T helper cells from peripheral blood), E044 (Primary T regulatory cells from peripheral blood), E045 (Primary T cells effector/memory enriched from peripheral blood), E047 (Primary T CD8 naïve cells from peripheral blood), E048 (Primary T CD8 memory cells from peripheral blood), E088 (Fetal lung), E096 (Lung), E114 (A549 EtOH 0.02pct Lung Carcinoma Cell Line), and E128 (NHLF Human Lung Fibroblast Primary Cells). The colors denote chromatin states imputed by ChromHMM, with the color key in the gray box (“Materials and methods”)

Multi-level functional annotations linked 3p21.31 locus with CXCR6 and CCR9 functions

To explore the potential functions linked with the GWAS risk variants, we examined the functional genomic annotations in this locus. Specifically, we found a consistent decreasing effect of CXCR6 expression in T cells and whole blood from the two large-scaled eQTL datasets (Fig. 3B). The Hi-C cell line data from lung fibroblast (Dixon et al. 2012) also showed a significant interaction between the 3p21.31 locus and both promoter regions of CXCR6 and CCR9 (Fig. 3C). Furthermore, multiple SNPs in the 3p21.31 locus reside in the annotated regulatory elements across blood, T cell, and lung cell lines (Fig. 3D, see “Materials and methods”). Overall, these results from the multiple lines of evidence all supported the potential regulatory effects of the 3p21.31 locus on CXCR6 and CCR9 expression.

CXCR6 differentially expressed in TRM cells of severe and moderate patients

According to our tissue cell-type-specific expression database (CSEA-DB), CXCR6 is mainly expressed in immune cells in human lung tissue (e.g., T cell and NK cell) (Dai et al. 2021). In Liao et al.’s work, the authors reported that CXCR6 had lower expression in severe patients than moderate patients, indicating a potential protective effect in T cells of human respiratory systems (Liao et al. 2020). However, T cells have various resident and circulating subtypes with diverse functions (Hombrink et al. 2016). To understand which subpopulation(s) of T cells might be associated with the severity of COVID-19, we used the BLAF scRNA-seq data of severe and moderate patients. The data included 2677 CD8+ T-cells (1,329 from five severe patients and 1349 from three moderate patients). We further used a set of 31 TRM cell marker genes to distinguish the TRM cells and conventional CD8+ T cells (see “Materials and methods”). As shown in Fig. 4A, B, the TRM cells and conventional T cells could be distinguished in both moderate and severe patients with the classic TRM cells markers (CXCR6 (Kumar et al. 2017), CD69 (Martin and Badovinac 2018), ITGAE (the gene encoding CD103) (Martin and Badovinac 2018; Wauters et al. 2021), ZNF683 (Wauters et al. 2021), and XCL1 (Hombrink et al. 2016)) and three negative-control markers (SELL (the gene encoding CD62L) (Martin and Badovinac 2018), KLF2, and S1PR1 (Skon et al. 2013)) from a previous study (Kumar et al. 2017). After removing the outlier cells and CD4 T cells (see “Materials and methods”), we obtained 1101 lung-resident TRM cells, including 765 cells from moderate patients and only 336 cells from severe patients. This represented a 2.24-fold decrease for the expected number of TRM cells in severe patients in CD8+ T cells (Table 2). We used the non-parametric Wilcoxon rank-sum test to identify the DEGs in the TRM cells between severe and moderate patients and found CXCR6 had significantly lower expression in the severe patients than the moderate patients (p < 2.2 × 10–16, fold change = 1.50, Fig. 4C). We further checked the CXCR6 expression in TRM cells by sample to exclude the possibility that CXCR6 DEG analyses were disrupted by excessively high or low expression of certain samples (Supplementary Table S4).

Fig. 4
figure4

Single-cell transcriptome analysis of the severe and moderate COVID-19 patients. a Relative expression of the lung-resident memory CD8+ T (TRM) signature genes in TRM cells and conventional CD8+ T cells in moderate patients. b Relative expression of the TRM featured genes in TRM cells and conventional CD8+ T cells in severe patients. c CXCR6 expression in the TRM cells of moderate and severe patients. We split the TRM cells from the annotation of the original paper with 31 marker genes (“Materials and methods”). We conducted a two-sided non-parameter Wilcoxon rank-sum test to test whether CXCR6 was differentially expressed in moderate (red) and severe (blue) groups of TRM cells. “***” indicates it is genome-wide significant after multiple-test correction of all expressed genes. The small points denote the normalized expression in each cell. Mean normalized expression of CXCR6 in each group is highlighted with the largest circle in black. d Pseudotime inference for the moderate and severe TRM cells. The red and blue points on t-Distributed Stochastic Neighbor Embedding (tSNE) projection denote the TRM cells from moderate and severe patients, respectively. The x-axis and y-axis are the first and second dimensions of the tSNE, respectively. e Relative expression of the CXCR6 and naïve and effector T cell markers along the pseudotime proportional to the green color. The gene expressions are scaled by cells. Cells from moderate and severe groups are annotated in blue and red. f Relevance score for hallmark pathways from the molecular signatures database (MSigDB) along the pseudotime. The relevance score (R2 coefficient of determination) indicates the proportion of variance in the pseudotime explained by the genes in the hallmark pathways. g Relevance score for transcription factors and their target genes along the pseudotime. The relevance score denotes the proportion of variance in the pseudotime explained by the target genes regulated by the transcription factor

Table 2 Counts and ratio of TRM cells in moderate and severe patients relevant to CD8+ T cells

Inferring the transcriptional programs that drive the cell status transition

Previous COVID-19 studies demonstrated that performing pseudotime analysis could infer a cellular trajectory that indicates the disease progression from scRNA-seq data (Bernardes et al. 2020; Jeong et al. 2021). Therefore, to understand the transition between moderate and severe TRM cells, we constructed the cellular trajectory/pseudotime along with TRM cells using Slingshot (Fig. 4D) (Street et al. 2018). Next, we applied our DrivAER approach (Driving transcriptional programs based on AutoEncoder derived Relevance scores) (Simon et al. 2020) to identify the potential transcriptional programs that were most likely involved in the cellular trajectory/pseudotime. Figure 4D shows a scaled heatmap to demonstrate the relative expression of naïve and effector markers of T cells in the order of pseudotime generated by Slingshot (Han et al. 2018; Street et al. 2018). We identified that the severe TRM cells were mainly gathered in the later stage of the pseudotime. The naïve markers (IL7R, BCL2) were higher expressed in moderate patients than in severe patients (except SELL). On the contrary, some effector markers (GZMB, HAVCR2, LAG3, IFNG) were lower expressed in moderate patients than in severe patients. Other effector markers (IRF4, PRF1) had higher expression in the middle of the transition than their expression at the start and end sides. Furthermore, we also found that there was a negative correlation between CXCR6 expression and the pseudotime (Pearson correlation coefficient \(\rho =-0.36\), Supplementary Figure S2). These results indicated the TRM cells in severe patients still in pro-inflammatory status although the TRM cells status was more heterogeneous in severe patients than in moderate patients (Fig. 4a, b, e). As shown in Fig. 4f, g, the top five molecular signatures (relevance score > 0.25) identified by DrivAER included T cell pro-inflammatory actions (interferon gamma response, allograft rejection (Benichou et al. 2017), and complement system) as well as apoptosis (Cizmecioglu et al. 2021) and hypoxia (Jahani et al. 2020) pathways. Among the top TFs (relevance score > 0.2) that drove this trajectory, the DNA-binding RELA-NFKB1 complex is involved in several biological processes, such as inflammation, immunity, and cell growth initiated by external stimuli. MYC gene is related to the cell cycle and apoptosis pathway and upstream transcription factor 1 (USF1) is related to metabolic alteration associated inflammation process (Ruuth et al. 2018). In summary, the TF results are well consistent with our previous hallmark pathway findings (Supplementary Table S5 and S6).

Several genome-wide significant SNPs might change the TF binding-site affinity

To understand the potential TF binding affinity changes of genome-wide significant SNPs, we conducted the DNA motif recognition analysis of the five TFs related to the transcriptional program between moderate and severe TRM cells (relevance score > 0.2, Supplementary Table S6). We identified SNP rs10490770 [T/C, minor allele frequency (MAF) = 0.097, GWASHGI = 9.53 × 10–39] and SNP rs67959919 (G/A, MAF = 0.097, GWASHGI = 8.83 × 10–39) that were predicted to alter the binding affinity of TFs RELA and SP1, respectively (Supplementary Fig. S3a and S3b). Moreover, these two SNPs were in the high LD region (r2 > 0.8) with several significant lead eQTLs (SNP rs35896106 and rs17713054) of CXCR6 in whole blood (p = 5.03 × 10–37) and T follicular helper cell (p = 1.30 × 10–5) (Fig. 3B). In summary, the genome-wide significant SNPs were predicted to change the binding affinity of those TFs highly related to TRM cells status transition, (Supplementary Table S6), suggesting their potential regulation of CXCR6 expression.

Discussion

In this work, we developed a multi-level, integrative genetic and functional analysis framework to explore the host genetic factors on the expression change of GWAS-implicated genes for COVID-19 severity. Specifically, we conducted TWAS analysis for two independent COVID-19 GWAS datasets. We identified and replicated three chemokine receptor genes, CXCR6 and CCR9, with a protective effect in the lung and a risk effect in whole blood, respectively. CXCR6 is expressed in T lymphocytes and essential genes in CD8+ TRM cells, mediating the homing of TRM cells to the lung along with its ligand CXCL16 (Payne et al. 2021; Wein et al. 2019). CCR9 was reported to regulate chemotaxis in response to thymus-expressed chemokines in T cells (Lee et al. 2012). The colocalization analysis identified that both GWAS and eQTLs of CXCR6 had high colocalization probabilities in the lung, whole blood, and T follicular helper cells, which confirms the genetic regulation roles at this locus. At the single-cell level, our DEG analysis identified CXCR6 gene had lower expression in the COVID-19 severe patients than the moderate patients in both T cells and TRM cells, supporting its protective effect identified in TWAS analysis in lung and whole blood. The expected proportion of TRM cells in CD8+ T cells also decreased by 2.24-fold (Table 2). We identified the major transition force from moderate TRM cells to severe TRM cells are pro-inflammatory pathways and TFs.

From the TWAS and colocalization analysis in lung and immune cells, we successfully replicated that CXCR6 was centered in the GWAS signal in the 3p21.31 locus. Previous studies have reported that CXCR6−/− significantly decreases airway lung TRM cells due to altered trafficking of CXCR6−/− cells within the lung of the mice (Wein et al. 2019), which could explain a much less proportion of TRM cells in severe patients than moderate patients. The lung TRM cells provide the first line of defense against infection and coordinate the subsequent adaptive response (Ardain et al. 2020). The previous study has reported that TRM cells constitutively expressed surface receptors (PD-1 and CTLA-4) that are associated with inhibition of T cell function, which might prevent excessive activation or inflammation in the tissue niche (Szabo et al. 2019).

We are aware of several recent works have been decoding the genetic factors in the 3p21.31 locus. In Pairo-Castineira et al. (Pairo-Castineira et al. 2020), their TWAS analyses highlighted CCR2, CCR3, and CXCR6 genes in using the Meta-TWAS method MultiXcan for all GTEx v8 MASHR prediction model. We successfully replicated their CXCR6 in lung for both HGI and SCGG cohorts and CCR3 in spleen for HGI cohort only. The function of CCR3 at the single-cell level remained investigated since there is virtually absent CCR3 expression in our BALF dataset. We failed to detect any significant TWAS signals for CCR2 in any tissues or cohorts. Differences in associations identified in our TWAS compared to the previous study could be due to different TWAS prediction models used and different GWAS sample compositions. We also identified several novel candidates CCR9, CCR1, CCR5, and CCRL2 that held a line of evidence from either TWAS or colocalization analysis (Supplementary Table S1, S2, S3).

We further explored the known function of CXCR6 associated with COVID-19 and other infectious diseases in case–control studies, phenome-wide association study (PheWAS) and GWAS. The association of the single-cell level phenotype (lower expression of CXCR6 and decreased proportion of CD8+ CXCR6+ T cells) and the severe COVID-19 has been observed in another work in circulating T cells with flow cytometry experiments (Payne et al. 2021), suggesting the significant role of the chemokine receptor CXCR6 and its ligand CXCL16 in the immunopathogenesis of severe COVID-19. Another recent CRISPR screen study also suggested CXCR6 is one of the top-ranked genes, whose loss reduces SARS-CoV-2 infection in lung epithelial-like cells (Kasela et al. 2021). Moreover, in Ashhurst et al.’s work (Ashhurst et al. 2019), they identified CXCR6 was redundant for T-lymphocyte recruitment to airway and lung. The CXCR6-deficiency mice could increase the host control of tuberculosis and influenza through other alternative receptors (CXCR3, CCR5, and CCR4) to activate the inflammatory response, suggesting that other activated pathways might compensate CXCR6-deficiency in COVID-19 severe patients. We applied PhenoScanncer to conduct the PheWAS analysis (Kamat et al. 2019) and identified several phenotypes “monocyte percentage of white cells” and “monocyte count” related to SNPs in CXCR6 gene regions, indicating potential association with CXCR6. However, those lead SNPs in CXCR6 might be cis-regulators of nearby genes due to the high linkage disequilibrium (LD) in this 3p21.31 locus. In GWAS, we failed to find any GWAS risk variants annotated to CXCR6 from the GWAS catalog using keywords “pulmonary tuberculosis”, “influenza”, “malaria” or “HIV” (Buniello et al. 2019).

To understand the status of TRM cells, we further used nine classic naïve markers (e.g., BCL2, SELL, TCF7, and IL7R) and ten classic effector markers (e.g., GZMB, PRF1, IFNG, LAG3, and PDCD1) to quantify the naïve and effector status of the TRM cells (Supplementary Figure S4). TRM cells in severe patients had a much higher median of effector marker score (0.48 in severe group and 0.19 in moderate group) than TRM cells in moderate patients did, suggesting that the severe TRM cells had much higher activities in inflammation as we discovered in Fig. 4F despite their proportion decrease. For the naïve score (Supplementary Figure S4), both moderate and severe TRM cells had limited expressions (median: 0.032 in the severe group; median: 0.027 in the moderate group). Interestingly, if we removed the lymph node homing receptor SELL (Kumar et al. 2017) from the naïve markers list, we would find the median naïve markers in the severe group would drop to 0 (Supplementary Figure S4). This indicated that SELL expression contributed greatly to the naïve status of TRM severe patients. Consistently in Fig. 4E, we could also observe that a large proportion of TRM cells had higher SELL expression in severe patients than in moderate patients, suggesting the TRM cells in severe patients might not be in a stable cell status due to the expression of lymph node homing signal (SELL). As illustrated in Fig. 5, we hypothesized that host genetic variants or other unknown factors would lead to lower expression of CXCR6, which might decrease the proportion of TRM cells residing in the lung through the CXCR6/CXCL16 axis (Payne et al. 2021; Wein et al. 2019), impairing the first-line defense. Moreover, the lower expression of CXCR6 might be related to the “unstable” residency of TRM cells in lung. Since TRM cells play essential roles in orchestrating the immune system, the lack of which would lead to severe COVID-19 symptoms, such as acute respiratory distress syndrome, cytokine storm, and major multi-organ damage (Tay et al. 2020). Lastly, the CXCR6-deficiency might also lead to the compensatory inflammatory response (Ashhurst et al. 2019).

Fig. 5
figure5

The proposed CXCR6 regulation mechanism on COVID-19 severity. We proposed one pathogenesis mechanism using current knowledge to explain how the lower expression of CXCR6 could be associated with the outcome of severe COVID-19 symptoms, which was supported by our findings of the genetic factors on decreasing the CXCR6 expression and aligned with our observations from single-cell transcriptome analysis. The star on the DNA indicates the host genetic effects or other unknown factors

The GWASHGI dataset used in this study was HGI round 4 (alpha), which was the largest GWAS by the access date of October 20, 2020. However, it was not the currently largest GWAS meta-analysis for severe COVID-19 when we prepared the manuscript. This research field is evolving very fast, due to the urgent demand for public health. Currently, the largest GWAS HGI round 4 (freeze) contained more samples (4336 cases/353,891 controls), and it included two independent datasets we used in this study. Considering that the GWASHGI dataset included ~ 10% control samples from the Asian population, we checked the LocusZoom plot of the chr3: 45.80–46.40 million base pairs (Mb) region on GRCh37 reference genome. We found a consistent tendency in GWAS round 4 alpha and freeze version (Supplementary Figure S5). Another limitation is that the scRNA-seq data only had eight COVID-19 patient samples (five severe and three moderate samples), which might not provide enough statistical power at the sample level as it is commonly considered each scRNA-seq data acts as a population. Thus, our single-cell findings remain further investigated in larger sample-size studies. Finally, the TF binding-site affinity alterations were assessed based on computational prediction, therefore, the in vivo effects require experimental validation. We anticipate more and larger datasets will be released in the near future. We will apply our integrative analysis approach to such new data.

Our work systematically explored the genetic effect on gene expression in the chromosome 3p21.31 locus and pinpointed the multi-evidence validated gene CXCR6 might be involved in the severity of COVID-19. Several genome-wide significant SNPs were within the LD block of CXCR6 eQTLs in immune-related cells. In a scRNA-seq COVID-19 BALF dataset, we characterized that CXCR6 (TRM cells marker gene) had a lower expression in severe patients than in moderate patients. Moreover, the TRM cells in severe patients had a 2.24-fold proportion decrease in CD8+ T cells and much higher pro-inflammatory activity than TRM cells in moderate patients. Based on these observations, we proposed a potential mechanism on how the lower expression of CXCR6 regulated by the endogenous factors or other unknown factors could progress to severe COVID-19 outcomes.

Data availability

All datasets analyzed in this study were publicly available. Other data could be accessed from public resources described in Methods.

Code availability

Available upon reasonable request.

Abbreviations

BALF:

Bronchoalveolar lavage fluid

BIOS:

Biobank-based Integrative Omics Studies

ChromHMM:

Chromatin-state hidden Markov model

COVID-19:

Coronavirus disease 2019

CLPP:

Colocalization posterior probability

CSEA-DB:

Cell-type-specific expression database

DAP:

Deterministic approximation of posteriors

DEG:

Differentially expressed gene

DICE:

Database of immune cell expression

DrivAER:

Driving transcriptional programs based on AutoEncoder derived Relevance scores

eQTL:

Expression quantitative trait

GReX:

Genetically regulated expression

GWAS:

Genome-wide association study

HGI:

Host Genetics Initiative

Hi-C:

High-throughput chromatin interaction

LD:

Linkage disequilibrium

MAF:

Minor allele frequency

MASHR:

Multivariate adaptive shrinkage in R

Mb:

Million base pairs

MSigDB:

Molecular signatures database

PIP:

Posterior inclusion probability

PWM:

Position weight matrix

SARS-CoV-2:

Severe acute respiratory syndrome coronavirus 2

RCP:

Regional colocalization probability

SCGG:

Severe COVID-19 GWAS Group

scRNA-seq:

Single-cell RNA sequencing

tSNE:

T-Distributed Stochastic Neighbor Embedding

TF:

Transcription factor

TRM cells:

Resident memory CD8+ T cells

TWAS:

Transcriptome-wide association study

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Acknowledgements

We appreciate Drs. Teng Liu and Dawei Zou for the valuable comments. We thank all members of the Bioinformatics and Systems Medicine Laboratory for the discussion.

Funding

Dr. Zhao was partially supported by National Institutes of Health grants (R01LM012806 and R01DE030122) and Chair Professorship for Precision Health funds. We thank the technical support from the Cancer Genomics Core funded by the Cancer Prevention and Research Institute of Texas (CPRIT RP180734). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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YD, ZZ, JW and WC contributed to the conception and design of the study; YD, JW, and HHJ collected the data and performed the analysis; YD, JW, WC, PJ and ZZ interpreted the results; YD, JW, HHJ, ZZ, and PJ wrote the manuscript. All authors read and approved the final manuscript.

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Correspondence to Zhongming Zhao.

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Dai, Y., Wang, J., Jeong, HH. et al. Association of CXCR6 with COVID-19 severity: delineating the host genetic factors in transcriptomic regulation. Hum Genet (2021). https://doi.org/10.1007/s00439-021-02305-z

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