Abstract
The minor haplotype −3575A/-2849G/-2763C in IL-10 promoter has been defined as a marker of disease resistance to leprosy and its severity in Brazilian population. Our investigation of six single-nucleotide polymorphisms (SNPs) in IL-10 promoter in 282 Indian leprosy patients and 266 healthy controls by direct PCR sequencing, however, showed that the extended haplotype: −3575T/-2849G/-2763C/-1082A/-819C/-592C was associated with resistance to leprosy per se and to the development of severe form of leprosy, using either a binomial (controls vs cases, P=0.01, OR=0.58, CI=0.37–0.89) or ordinal (controls vs paucibacillary vs multibacillary, P=0.004) model. Whereas, IL-10 haplotype −3575T/-2849G/-2763C/-1082A/-819T/-592A was associated with the risk of development of severe form of leprosy (P=0.0002) in contrast to the minor risk haplotype −3575T/-2849A/-2763C in the Brazilian population. The role of IL-10 promoter SNPs in Brazilian and Indian population strongly suggests the involvement of IL-10 locus in the outcome of leprosy.
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Acknowledgements
We are grateful to Prof. Jason P. Sinnwell for his invaluable support in providing Haplo.Stats package instructions and statistical analysis. Financial support provided to the National Centre of Applied Human Genetics by the University Grants Commission (New Delhi, India), Grant number: F17-4/2001/(NEW SCHEME/COS1) is duly acknowledged. The authors acknowledge the infrastructural support provided by the Centre for Advanced Studies (funded by UGC), School of Life Sciences, JNU, New Delhi.
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Malhotra, D., Darvishi, K., Sood, S. et al. IL-10 promoter single nucleotide polymorphisms are significantly associated with resistance to leprosy. Hum Genet 118, 295–300 (2005). https://doi.org/10.1007/s00439-005-0042-8
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DOI: https://doi.org/10.1007/s00439-005-0042-8