Molecular and General Genetics MGG

, Volume 259, Issue 3, pp 256–263 | Cite as

Structure of the Chlorella Zepp retrotransposon: nested Zepp clusters in the genome

  • Y. Noutoshi
  • R. Arai
  • M. Fujie
  • T. Yamada
ORIGINAL PAPER

Abstract

Zepp elements found in the telomeric region of Chlorella chromosomes show the characteristic features of non-viral (LINE-like) retrotransposons, including a poly(A) tail, 5′ truncations, a retroviral reverse transcriptase-like ORF and flanking target duplications. We have isolated and characterized a full-length Zepp element (8943 bp long) from Chlorella chromosome V. Some peculiar features of this element, including nested integration, two ORF structures, a long 3′ noncoding region and a possible promoter region are compared with those of the Drosophila telomeric retrotransposons HeT-A and TART. The Chlorella chromosome-Zepp system appears to represent an intermediate stage between canonical telomerase-telomeres and Drosophila retrotransposon-telomeres.

Key wordsChlorella LINE-like retrotransposon Nested integration Full-length copy Long 3′ noncoding region 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • Y. Noutoshi
    • 1
  • R. Arai
    • 1
  • M. Fujie
    • 1
  • T. Yamada
    • 1
  1. 1.Department of Molecular Biotechnology, Graduate School of Engineering, Hiroshima University, Kagamiyama 1–4-1, Higashi-Hiroshima 739-8527 Japan. Fax: +81-824-247752; e-mail: tayamad@ipc.hiroshima-u.ac.jpJP

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