Abstract
The genes encoding creatininase (CrnA; 258 residues) and creatinase (CreA; 411 residues) from Arthrobacter sp. TE1826 were cloned and sequenced. The genes form a cluster with the sarcosine oxidase gene (soxA) and its regulator gene (soxR), which were cloned previously. The deduced amino acid sequences of CrnA and CreA show 35.9% and 63.1% identity, respectively to the corresponding Pseudomonas enzymes. CrnA and CreA were purified from the recombinant strains and characterized. Other open reading frames (creB and crnB), encoding proteins similar to several transporters, were found downstream of creA and crnA, respectively.
Similar content being viewed by others
Author information
Authors and Affiliations
Additional information
Received: 15 July 1997 / Accepted: 20 October 1997
Rights and permissions
About this article
Cite this article
Nishiya, Y., Toda, A. & Imanaka, T. Gene cluster for creatinine degradation in Arthrobacter sp. TE1826. Mol Gen Genet 257, 581–586 (1998). https://doi.org/10.1007/s004380050685
Issue Date:
DOI: https://doi.org/10.1007/s004380050685