Structure and function of the vp1 gene homologue from the resurrection plant Craterostigma plantagineum Hochst.

Abstract

A gene that is homologous to the vp1 genes from maize and rice, the Arabidopsis abi3 gene and PvAlf from Phaseolus vulgaris was isolated from the resurrection plant, Craterostigma plantagineum Hochst. The C. plantagineum gene (cpvp1) encodes a protein of 688 amino acids and contains five introns at positions identical to those in the Arabidopsis, maize and rice homologues. The cpvp1 transcript is present in mature seeds and in young seedlings up to 8 days following germination. The ability of the cpvp1 gene product to activate target genes was demonstrated by transient expression experiments in tobacco protoplasts using reporter gene constructs containing the β-glucuronidase (GUS) gene fused to the promoter of two late embryogenesis abundant (LEA)-like genes, CDeT27-45 and CDeT6-19 from C. plantagineum, or the promoter of a maize gene encoding a 22-kDa zein seed storage protein.