Abstract
TSD2, a gene necessary for DNA synthesis in Ustilago maydis, was cloned by complementation of the temperature sensitive growth defect of a mutant known previously as pol1-1 and renamed here tsd2-1. Linkage analysis established that the cloned fragment contained an allele of tsd2-1 and not a suppressor. DNA sequence determination of the cloned DNA fragment indicated the presence of a single large uninterrupted open reading frame capable of encoding a protein of 845 amino acids without homology to any known gene involved in DNA synthesis. TSD2 was found to be cell cycle-regulated and mRNA levels peaked in early S or G1 phase.
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Received: 27 March 1996 / Accepted: 28 August 1996
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Onel, K., Holloman, W. Molecular cloning of a gene required for DNA replication in Ustilago maydis . Mol Gen Genet 253, 463–468 (1997). https://doi.org/10.1007/s004380050344
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DOI: https://doi.org/10.1007/s004380050344