Skip to main content
SpringerLink
Log in

Sequence comparisons among dispersed members of the Brassica S multigene family in an S 9 genome

  • Original Paper
  • Published:
Molecular Genetics and Genomics Aims and scope Submit manuscript

Abstract.

Self-incompatibility (SI) systems prevent self-pollination and promote outbreeding. In Brassica, the SI genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are members of the S multigene family, which share the SLG-like domain (S domain), which encodes a putative receptor. We have cloned members of the S multigene family from the S 9 haplotype of B. campestris (syn. rapa). In addition, eight distinct genomic regions harboring 10 SLG/SRK-like genes were characterized in the present study. Sequence analysis revealed two novel SRK-like genes, BcRK3 and BcRK6 (for B. campestris receptor kinases 3 and 6, respectively). Other genes that were characterized included SFR2 (for S gene family receptor 2), SLR2 (for S locus related gene 2), and a pseudogene. Based on phylogenetic analysis of the nucleotide sequences of the S domain regions, SLG and SRK appear to be distinct from other members of the S multigene family. Linkage analysis showed that most members of the S multigene family are dispersed in the Brassica genome, and that SLR1 (S locus related gene 1) is not linked to the SLR2 in B. campestris.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Author notes

  1. N. Kai

    Present address: Present address: Life Science Laboratory, Mitsui Chemicals Inc., Mobara, Chiba 297-8666, Japan

  2. G. Suzuki

    Present address: Present address: Division of Natural Science, Osaka Kyoiku University, Kashiwara, Osaka 582-8582, Japan

Authors and Affiliations

  1. Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan

    N. Kai, G. Suzuki & M. Watanabe

  2. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0101, Japan

    A. Isogai

  3. Research Institute of Seed Production Company Ltd., Sendai 989-3204, Japan

    K. Hinata

  4. Laboratory of Plant Breeding, Faculty of Agriculture, Iwate University 3-18-8, Ueda, Morioka 020-8550, Japan

    M. Watanabe

Authors
  1. N. Kai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Suzuki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Isogai
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. Hinata
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Electronic Publication

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kai, N., Suzuki, G., Watanabe, M. et al. Sequence comparisons among dispersed members of the Brassica S multigene family in an S 9 genome. Mol Gen Genomics 265, 526–534 (2001). https://doi.org/10.1007/s004380000441

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s004380000441

Search

Navigation