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The ITR binding domain of the Mariner Mos-1 transposase

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Abstract

Mariner-like elements are widespread eukaryotic transposons, but Mos-1 is the only natural element that is known to be active. Little is known about the biochemistry of mariner transposition. The first step in the process is the binding of the transposase to the 5′ and 3′ inverted terminal repeats (ITRs) of the element. Using the 3′ ITR of the element, we have determined the binding properties of a recombinant Mos-1 transposase produced in bacteria, and we have used deletion derivatives to localize the minimal ITR binding domain between amino acids 1 and 141. Its features and structure indicate that it differs from the ITR binding domain of the transposase encoded by Tc1-related elements.

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Augé-Gouillou, C., Hamelin, MH., Demattei, MV. et al. The ITR binding domain of the Mariner Mos-1 transposase. Mol Gen Genomics 265, 58–65 (2001). https://doi.org/10.1007/s004380000386

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  • DOI: https://doi.org/10.1007/s004380000386

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