Abstract.
The SeqA protein acts as a regulator of chromosomal replication initiation in Escherichia coli by sequestering hemi-methylated oriC, effectively blocking methylation and therefore preventing rapid re-initiation. The level of SeqA protein is maximal at mid-log phase and decreases when cells enter late-log phase. In hup mutants that lack the HU protein, the maximal seqA expression is also seen at mid-log phase, but seqA expression, as well as SeqA levels and activity, is increased by up to four fold relative to that in the wild type. These results suggest that the HU protein functions as a negative modulator of seqA expression.
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lee, H., Kim, H., Kang, S. et al. Expression of the seqA gene is negatively modulated by the HU protein in Escherichia coli . Mol Gen Genet 264, 932–936 (2001). https://doi.org/10.1007/s004380000384
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s004380000384