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Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis

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Abstract.

When expressed in Escherichia coli, the AddAB exonuclease/recombinase from Bacillus subtilis blocks the growth of phage λ. Mutants of λ that are deleted for ea47, a gene of unknown function which is expressed early in the lytic cycle, are not blocked for growth. The blocked-growth phenotype of λ ea47 + in the presence of AddAB is expressed only when phage DNA replication is permitted.

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Stahl, F., Bowers Jr., R., Mooney, D. et al. Growth and recombination of phage λ in the presence of exonuclease V from Bacillus subtilis . Mol Gen Genet 264, 716–723 (2001). https://doi.org/10.1007/s004380000361

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  • DOI: https://doi.org/10.1007/s004380000361

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