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Signature and function of plasma exosome-derived circular RNAs in patients with hypertensive intracerebral hemorrhage

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Abstract

Intracerebral hemorrhage (ICH) is one of the major causes of death and disability, and hypertensive ICH (HICH) is the most common type of ICH. Currently, the outcomes of HICH patients remain poor after treatment, and early prognosis prediction of HICH is important. However, there are limited effective clinical treatments and biomarkers for HICH patients. Although circRNA has been widely studied in diseases, the role of plasma exosomal circRNAs in HICH remains unknown. The present study was conducted to investigate the characteristics and function of plasma exosomal circRNAs in six HICH patients using circRNA microarray and bioinformatics analysis. The results showed that there were 499 differentially expressed exosomal circRNAs between the HICH patients and control subjects. According to GO annotation and KEGG pathway analyses, the targets regulated by differentially expressed exosomal circRNAs were tightly related to the development of HICH via nerve/neuronal growth, neuroinflammation and endothelial homeostasis. And the differentially expressed exosomal circRNAs could mainly bind to four RNA-binding proteins (EIF4A3, FMRP, AGO2 and HUR). Moreover, of differentially expressed exosomal circRNAs, hsa_circ_00054843, hsa_circ_0010493 and hsa_circ_00090516 were significantly associated with bleeding volume and Glasgow Coma Scale score of the subjects. Our findings firstly revealed that the plasma exosomal circRNAs are significantly involved in the progression of HICH, and could be potent biomarkers for HICH. This provides the basis for further research to pinpoint the best biomarkers and illustrate the mechanism of exosomal circRNAs in HICH.

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Data available on request from the authors.

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (82171295), Chengdu Science and Technology Bureau (2020-GH02-00057-HZ), Sichuan Science and Technology Program (2023YFS0042, 2023NSFSC1462, 2021YFS0376), and Technology Department of Sichuan Province (2022NSFSC1560).

Funding

This study was supported by grants from the National Natural Science Foundation of China (82171295), Chengdu Science and Technology Bureau (2020-GH02-00057-HZ), Sichuan Science and Technology Program (2023YFS0042, 2023NSFSC1462, 2021YFS0376), and Technology Department of Sichuan Province (2022NSFSC1560).

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  1. Kejie Chen and Xiaoyuan Cheng contributed equally to this work.

Authors and Affiliations

  1. School of Public Health, Chengdu Medical College, Chengdu, Sichuan, 610500, People’s Republic of China

    Kejie Chen

  2. Department of Emergency, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, 610500, People’s Republic of China

    Xiaoyuan Cheng, Yang Sun & Shuping Li

  3. Department of Critical Care Medicine, The General Hospital of Western Theater Command, Chengdu, Sichuan, 610500, People’s Republic of China

    Shanshan Yuan

  4. School of Bioscience and Technology, Chengdu Medical College, Chengdu, Sichuan, 610500, People’s Republic of China

    Junli Hao

  5. Department of Neurology, The First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, 610500, People’s Republic of China

    Quandan Tan & Yapeng Lin

  6. Department of Neurology, Sichuan Provincial People’s Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, 610072, People’s Republic of China

    Jie Yang

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  1. Kejie Chen
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Contributions

Study conception and design were performed by Shuping Li and Jie Yang. All authors contributed to material preparation, data collection and analysis. The first draft of the manuscript was written by Kejie Chen and Xiaoyuan Cheng and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Shuping Li or Jie Yang.

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The authors have no competing interests to declare that are relevant to the content of this article.

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This study was performed in accordance with the ethical standards as laid down in the 1964 Declaration of Helsinki, and approved by the Ethics Committee of the First Affiliated Hospital of Chengdu Medical College (approval number: 2022CYFYIRB-BA-Apr04), and informed consent was obtained from the participants or legal representative.

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Communicated by Wenfei Jin.

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Chen, K., Cheng, X., Yuan, S. et al. Signature and function of plasma exosome-derived circular RNAs in patients with hypertensive intracerebral hemorrhage. Mol Genet Genomics 299, 50 (2024). https://doi.org/10.1007/s00438-024-02144-3

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