Abstract
Polymorphism of transcription factor 7-like 2 (TCF7L2) has a link with type 2 diabetes mellitus (T2DM) through β cell dysfunction that causes defect in blood glucose homeostasis. This case–control study recruited 67 T2DM as cases and 65 age-matched healthy individuals as controls to determine whether the polymorphism rs12255372 (G > T) in the TCF7L2 gene have an association with T2DM in Bangladeshi population. Genomic DNA was purified from peripheral whole blood sample and direct Sanger sequencing was done for genotyping of SNP. Bivariate logistic regression was done to find out the association between genetic variant and T2DM. In our study, the minor T allele frequency was significantly more frequent in T2DM group than healthy controls (29.1% vs. 16.9%). After adjusting with confounding factors, heterozygous-genotype GT had higher odds of developing T2DM (OR 2.4; 95% CI: 1.0–5.5; p value = 0.04) and in dominant model, having SNP in TCF7L2 increased the risk of T2DM 2.3 times (95% CI: 1.0–5.2; p value = 0.04). In interaction model, genetic susceptible SNP cases interacted significantly with increasing age and BMI, female gender, and having family history of diabetes mellitus to develop T2DM (pinteraction < 0.001). Having minor T allele either in heterozygous or homozygous variant form of rs12255372 (G > T) TCF7L2 had significant association with T2DM. In conclusion, TCF7L2 gene variant increases risk of developing T2DM among the Bangladeshi population.
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Data availability
The data underlying this article are available in the article and its online supplementary material (https://doi.org/10.17632/fmf9r9hhmr.2). Fasta sequence files/chromatograms are available upon request.
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Acknowledgements
This study received partial funding support from the Ministry of Science and Technology, Government of Bangladesh. We are also thankful to Santi Sir Memorial Diabetic Center of Sirajdikhan, Munshiganj for assisting us in data collection. We also thank Zubair Ahmed Ratan, Department of Biomedical Engineering, Khulna University of Engineering and Technology, Bangladesh, for critical reviewing of the manuscript and helpful suggestion.
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ABMMKI and NB conceived the project. NB, MF, MAY collected the data. NB and MAI performed statistical analysis. NB performed genetic analysis. NB drafted the manuscript. ABMMKI and MAI supervised the study. The manuscript was reviewed and approved by all authors.
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This study design was approved from the Ethical Review Committee at the Faculty of Biological Sciences of University of Dhaka, Bangladesh (Ref. No. 152/Biol. Scs. 2021). We obtained informed written consent from all the candidates who participated in this study. At the same time, we followed all the principles of Helsinki Declaration.
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Barman, N., Atiqul Haque, M., Firoz, M. et al. Association of transcription factor 7-like 2 rs12255372 polymorphism with susceptibility of type 2 diabetes mellitus in Bangladeshi population. Mol Genet Genomics 298, 1201–1209 (2023). https://doi.org/10.1007/s00438-023-02049-7
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DOI: https://doi.org/10.1007/s00438-023-02049-7