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Deletions in GSN gene associated with growth traits of four Chinese cattle breeds

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Abstract

The aim of this study was to assess the potential of 21 bp mutation in the second intron of the GSN gene as a molecular marker-assisted by exploring the effect of 21 bp mutation on growth traits in four beef cattle breeds. Gelsolin (GSN), a member of the superfamily of gel proteins, is involved in the regulation of a variety of cellular activities in the organism and plays an important function in cell motility, apoptosis, signal transduction and inflammatory responses. Gelatin can not only negatively regulate the pro-apoptotic effect of P53 protein, but also promote apoptosis by blocking the interaction between actin and deoxyribonuclease, so, the GSN gene was selected as a candidate gene in this study. In this study, a 21 bp mutation on the second intron to the GSN gene was verified in 573 individuals of Yunling (YL, n = 220), Jiaxian (JX, n = 140), Xianan (XN, n = 114) and Qinchuan (QC, n = 97) cattle breeds using Once PCR and agarose gel electrophoresis. The association analysis of polymorphisms in the GSN gene with growth traits in four breeds was revealed: in YL cattle, the heart girth and forehead size of heterozygous ID genotype were significantly higher than II genotype (P < 0.05). In JX cattle, the withers height, body length and heart girth of II and ID genotype were significantly highest than DD genotype (P < 0.01); the height at hip cross and height at sacrum of II genotype was significantly highest than DD genotype (P < 0.01), but ID genotype was significantly higher than DD genotype. In XN cattle, the abdominal girth and circumference of the cannon bone of II genotype were significantly higher than ID genotype (P < 0.05). In QC cattle, the hucklebone width of ID genotype was significantly the highest than II genotype (P < 0.01). The results suggest that GSN may be an important candidate gene and that a 21 bp mutation on the second intron to the GSN gene can be used for molecular marker-assisted selection of four beef cattle breeds.

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Acknowledgements

This work was supported by the Program of National Beef Cattle and Yak Industrial Technology System (no. CARS-37), the Program of Yunling Scholar and the Young and Middle-aged Academic Technology Leader Backup Talent Cultivation Program in Yunnan Province, China (no. 2018HB045), Yunnan Provincial Major S&T Project (2019ZG007, 2019ZG011). The Agricultural Improved Seed Project of Shandong Province (2020LZGC014-03).

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Authors and Affiliations

  1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Ao Qi, Wenxiu Ru, Yangkai Liu, Yu Yang, Jia Tang, Haiyan Yang, Shuling Yang, Xianyong Lan, Chuzhao Lei & Hong Chen

  2. Academy of Science and Technology, Chuxiong Normal University, Chuxiong, 675000, Yunnan, China

    Kaixing Qu

  3. Yunnan Academy of Grassland and Animal Science, Kunming, 650212, Yunnan, China

    Bizhi Huang

  4. College of Animal Science, Xinjiang Agricultural University, Urumqi, 830052, China

    Hong Chen

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  1. Ao Qi
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  2. Wenxiu Ru
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Correspondence to Bizhi Huang or Hong Chen.

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Qi, A., Ru, W., Liu, Y. et al. Deletions in GSN gene associated with growth traits of four Chinese cattle breeds. Mol Genet Genomics 297, 1269–1275 (2022). https://doi.org/10.1007/s00438-022-01915-0

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