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Characteristics of the TDRD1 gene promoter in chickens

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Abstract

Tudor domain containing 1 (TDRD1) is a member of the TDRD family and plays an important role in embryogenesis and gametogenesis. A detailed study of the characteristics of chicken TDRD1 can lay a foundation for the study of chicken spermatogonia stem cell formation and spermatogenesis. We cloned 2117 bp upstream fragment of TDRD1 promoter and constructed a series of recombinant vectors with different length deletions. The dual-luciferase experiments reveal that the upstream region of – 161 to 0 bp was its core transcription promoter region. Bioinformatics analysis predicted the possible binding of Transcription Factor 7 Like 2 (TCF7L2) and Zinc Finger E-Box-Binding Homeobox 1(ZEB1) transcription factors in the core region. The transcriptional activity of TDRD1 was significantly decreased after mutation of TCF7L2-binding site, while that of TDRD1 was significantly increased after mutation of ZEB1-binding site. Further, ChIP experiments verified that TCF7L2 enriched in the TDRD1 core transcriptional initiation region, suggesting that TCF7L2 and ZEB1 play an important role in the regulation of TDRD1. In summary, the region from – 161 to 0 bp is the core promoter region of TDRD1; ZEB1 and TCF7L2 bind to the TDRD1 promoter region and TCF7L2 activates the transcription of TDRD1 gene.

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Acknowledgements

The authors gratefully acknowledge the financial support for this work from the National Natural Science Foundation of China (31972547, 31772582, 32002158), Key Research and Development Program (2017YFE0108000) and China Postdoctoral Science Foundation (2020M681746). Finally, we are very grateful to our colleagues and teachers in the laboratory for their mutual help.

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Author notes

  1. Xiaomin Gao, Xiaolin Sun and Pei Xu contributes equally to this work.

Authors and Affiliations

  1. Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, 225009, China

    Xiaomin Gao, Xiang Shi, Ming Zhang, Xia Yuan, Jingyi Jiang, Kai Jin, Qisheng Zuo, Yani Zhang & Bichun Li

  2. Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou, 225009, China

    Xiaomin Gao, Xiang Shi, Ming Zhang, Xia Yuan, Jingyi Jiang, Kai Jin, Qisheng Zuo, Yani Zhang & Bichun Li

  3. Key Laboratory of Animal Breeding Reproduction and Molecular Design for Jiangsu Province, College of Animal, Yangzhou University, Yangzhou, 225009, China

    Xiaomin Gao, Xiang Shi, Ming Zhang, Xia Yuan, Jingyi Jiang, Kai Jin, Qisheng Zuo, Yani Zhang & Bichun Li

  4. The People’s Hospital of Taizhou, Taizhou, Jiangsu, China

    Xiaolin Sun & Pei Xu

  5. Institute of Translational Medicine, Medical College, Yangzhou University, Yangzhou, Jiangsu, China

    Xiaolin Sun & Pei Xu

Authors
  1. Xiaomin Gao
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  2. Xiaolin Sun
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  3. Pei Xu
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  4. Xiang Shi
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  5. Ming Zhang
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  8. Kai Jin
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  9. Qisheng Zuo
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  10. Yani Zhang
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  11. Bichun Li
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Contributions

XG, PX, XS, BL and QZ conceived and designed the study and contributed to the analysis and interpretation of data. XG, XS, PX, XS, MZ, XY, JJ and YZ were involved in drafting the manuscript and revising it for important intellectual content. QZ helped with the speech correction. All authors reviewed and approved the final manuscript.

Corresponding author

Correspondence to Bichun Li.

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No conflict of interest exists in the submission of this article, and this article is approved by all authors for publication.

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Communicated by Joan Cerda.

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Gao, X., Sun, X., Xu, P. et al. Characteristics of the TDRD1 gene promoter in chickens. Mol Genet Genomics 297, 903–910 (2022). https://doi.org/10.1007/s00438-022-01886-2

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  • DOI: https://doi.org/10.1007/s00438-022-01886-2

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