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Isolation and characterization of SGF3, a novel Microviridae phage infecting Shigella flexneri

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Abstract

In the context of widespread bacterial contamination and the endless emergence of antibiotic-resistant bacteria, more effective ways to control pathogen infection are urgently needed. Phages become potential bactericidal agents due to their bactericidal specificity and not easy resistance to bacteria. But an important factor limiting its development is the lack of phage species. Therefore, the isolation of more new phages and studying their biological and genomic characteristics is of great significance for subsequent applications. So, in this study, SGF3, a Microviridae phage, which has shown lytic activity against Shigella flexneri, was isolated, purified, and characterized. Morphological and phylogenetic analyses identified it as a phiX174 species belonging to the Microviridae family. The latent period of phage SGF3 was 20 min, with an average burst size of approximately 7.1. Host spectrum experiments indicated its strong host specificity. Furthermore, the biofilm removal efficiency was increased by 20%-25% when SGF3 was coupled with other phages. In conclusion, the phage SGF3 found in this study was a lytic phage belonging to the Microviral family, and could be added as an auxiliary material in the phage cocktail. Studies of its characteristics and bactericidal properties had enriched the germplasm resources of microphages, provided more potential material in fighting against emerging and existing multidrug-resistant bacteria.

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Data availability

All data generated or analyzed during this study are included in this published article.

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All software or custom code used during this study are available.

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Acknowledgements

The authors would like to acknowledge the Institute of Microbiology, Chinese Academy of Sciences for electron microscopy.

Funding

This work was supported by the National Natural Science Foundation of China [No. 51378485].

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Shandong University, Jinan, 250100, China

    Han Lu

  2. College of Resources and Environment, University of Chinese Academy of Sciences, Beijing, 101408, China

    Han Lu, Wenbin Xiong, Zong Li, Peihan Yan, Ruyin Liu & Xinchun Liu

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  1. Han Lu
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  2. Wenbin Xiong
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  3. Zong Li
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  4. Peihan Yan
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  6. Xinchun Liu
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Contributions

LH: Performed the experiments, analyzed the data and wrote the first draft of the paper. LXC: Helped design the experiment. XWB: Assisted in experiments. LZ: Reviewed the paper. YPH: Further reviewed and edited. LRY: Commented on article revisions. All authors had read and approved the manuscript.

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Correspondence to Xinchun Liu.

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Lu, H., Xiong, W., Li, Z. et al. Isolation and characterization of SGF3, a novel Microviridae phage infecting Shigella flexneri. Mol Genet Genomics 297, 935–945 (2022). https://doi.org/10.1007/s00438-022-01883-5

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  • DOI: https://doi.org/10.1007/s00438-022-01883-5

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