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Genetic dissection of stalk lodging-related traits using an IBM Syn10 DH population in maize across three environments (Zea mays L.)

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Abstract

Stalk lodging severely limits the grain yield of maize (Zea mays L.). Mechanical stalk strength can be reflected by the traits of stalk diameter (SD), stalk bending strength (SBS), and lodging rind penetrometer resistance (RPR). To determine the genetic basis of maize stalk lodging, quantitative trait loci (QTLs) were mapped for these three traits using the IBM Syn10 DH population in three environments. The results indicated that there were strong genetic correlations among the three traits, and the analyses of phenotypic variations for SD, SBS, and RPR across the three environments showed high broad-sense heritability (0.6843, 0.5175, and 0.7379, respectively). In total, 44 significant QTLs were identified control the above traits across the 3 environments. A total of 14, 14, and 16 QTLs were identified for SD, SBS, and RPR across single-environment mapping, respectively. Notably, ten QTLs were stably expressed across multiple-environments, including two QTLs for SD, three for SBS, and five for RPR. Three major QTLs each accounting for over 10% of the phenotypic variation were qSD6-2 (10.03%), qSD8-2 (13.73%), and qSBS1-2 (11.89%). Comprehensive analysis of all QTLs in this study revealed that 5 QTL clusters including 12 QTLs were located on chromosomes 1, 3, 7, and 8, respectively. Among these 44 QTLs, 9 harbored 13 stalk lodging-associated SNPs that were detected by our recently published work, with 1 SNP successfully validated in the IBM Syn10 DH population. These chromosomal regions will be useful for marker-assisted selection and fine mapping of stalk lodging-related traits in maize.

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Acknowledgements

This work was supported by Major Project of Transgenic New Variety Cultivation (2016ZX08003003) and The National Natural Science Foundation of China (31871637).

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  1. Yanling Zhang and Tianhu Liang contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region, Maize Research Institute, Sichuan Agricultural University, Chengdu, 611130, China

    Yanling Zhang, Tianhu Liang, Min Chen, Yinchao Zhang, Tao Wang, Haijian Lin, Tingzhao Rong, Chaoying Zou, Peng Liu, Guangtang Pan & Yaou Shen

  2. Department of Agronomy, Iowa State University, Ames, IA, 50011, USA

    Michael Lee & Thomas Lübberstedt

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  1. Yanling Zhang
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Contributions

YS, GP, TR, TL, ML, and TW designed the experiments. YZ, THL, MC, ZZ, HL, GY, SZ, and CZ performed the analysis. YZ, YS, HP, and PL drafted the manuscript. All the authors critically revised and approval of the final version of this manuscript.

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Correspondence to Guangtang Pan or Yaou Shen.

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The authors declare that the research was conducted in the absence of any commercial or financial relationship that could be construed as a potential conflict of interest.

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This study does not include human or animal subjects.

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Communicated by S. Hohmann.

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Zhang, Y., Liang, T., Chen, M. et al. Genetic dissection of stalk lodging-related traits using an IBM Syn10 DH population in maize across three environments (Zea mays L.). Mol Genet Genomics 294, 1277–1288 (2019). https://doi.org/10.1007/s00438-019-01576-6

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