Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding

Reinke, Russell; Kim, Suk-Man; Kim, Bo-Kyeong

doi:10.1007/s00438-018-1470-1

Original Article
Published: 05 July 2018

Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding

Molecular Genetics and Genomics volume 293, pages 1565–1575 (2018)Cite this article

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Abstract

Yield losses as a result of biotic stresses by fungi, bacteria, viruses, and insects are a key challenge in most rice cultivation areas. The development of resistant cultivars is considered an efficient and sustainable approach to mitigate rice yield reduction. In the present study, we describe the development of japonica rice introgression lines with multiple resistance genes (MR lines), resistant to four different types of biotic stresses, and compare the agronomic performance, yield, and grain quality parameters of these lines with those of the recurrent parent. A total of nine MR lines were developed by marker-assisted backcrossing, which combined five single-R genes in a japonica background with a minimum of linkage drag. All the MR lines harbored the R genes Bph18 and qSTV11^SG and two Pi genes (Pib + Pik) in common, offering resistance to brown planthopper (BPH), rice stripe virus (RSV), and rice blast disease, respectively. In the case of bacterial blight (BB), Xa40 was detected in only five out of the nine and Xa3 was validated in the others. In particular, the five MR lines pyramiding the R genes (Bph18 + qSTV11SG + Pib + Pik) in combination with Xa40 showed stable resistance to all bioassays for BPH, BB, blast, and RSV. The MR lines did not show any negative effects on the main agronomic traits, including yield production and rice grain quality. The lines have significant potential to stabilize rice yield and minimize production costs in disease and pest-prone areas in Korea, through the pyramiding of five R genes using a marker-assisted backcrossing strategy.

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Abbreviations

ADV:: Alkali digestion value
BB:: Disease bacterial blight
BPH:: Brown planthopper
CL:: Culm length
DH:: Days to heading
GW:: Grain weight (GW)
MAS:: Marker-assisted selection
NGP:: Number of filled grains per panicle
NP:: Number of panicles
PL:: Panicle length
RP:: Recurrent parent
RGSV:: Rice grassy stunt virus
RRSV:: Rice ragged stunt virus
RSV:: Rice stripe virus
SS:: Seed set rate
Xoo:: Xanthomonas oryzae pv. oryzae

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Acknowledgements

This research was based on germplasm developed by Dr. K.K. Jena and was supported in part by a grant to the Agenda Program (Code No. PJ00926202), the Temperate Rice Research Consortium (TRRC) of the Rural Development Administration, Republic of Korea, and the Global Rice Science Partnership (GRiSP) program of IRRI. We thank the IRRI communication team for carefully editing the manuscript.

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Affiliations

Strategic Innovation Platform, International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines
Russell Reinke & Suk-Man Kim
C/o IRRI-Korea Office, National Institute of Crop Science, Rural Development Administration, Iseo-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea
Suk-Man Kim
National Institute of Crop Science, Rural Development Administration, Iseo-myeon, Wanju-gun, Jeollabuk-do, 55365, Republic of Korea
Bo-Kyeong Kim

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Russell Reinke
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Suk-Man Kim
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Bo-Kyeong Kim
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Correspondence to Russell Reinke.

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Communicated by S. Hohmann.

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Reinke, R., Kim, SM. & Kim, BK. Developing japonica rice introgression lines with multiple resistance genes for brown planthopper, bacterial blight, rice blast, and rice stripe virus using molecular breeding. Mol Genet Genomics 293, 1565–1575 (2018). https://doi.org/10.1007/s00438-018-1470-1

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Received: 28 September 2017
Accepted: 29 June 2018
Published: 05 July 2018
Issue Date: December 2018
DOI: https://doi.org/10.1007/s00438-018-1470-1

Keywords

Multiple resistance genes
Biotic stress
Gene pyramiding
Japonica rice
Marker-assisted selection