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RNA-Seq analysis reveals the distinctive adaxial–abaxial polarity in the asymmetric one-theca stamen of Canna indica

Molecular Genetics and Genomics volume 293, pages 391–400 (2018)Cite this article

Abstract

Proper establishment of adaxial–abaxial polarity is essential for the development of lateral organs, while former researches were mostly focused on the polarity regulation in leaves, and little is known in stamens, especially in the asymmetric ones. Canna indica (Zingiberales: Cannaceae) is a widely cultivated ornamental plant and the representative species to study the evolutionary development of Zingiberales. The androecium of Canna indica comprises 3–4 petaloid staminodes and a fertile stamen (FS), which consists of a one-theca anther and a petaloid appendage. The partially petaloid stamen is considered as an intermediate state organ from a two-thecae stamen to a completely petaloid staminode. Using RNA-Seq, we quantified the expressions of the transcripts in anther and petaloid appendage, and detected 64,430 and 57,041 unigenes in these two organs, respectively. 4574 unigenes were down-regulated, and 3525 were up-regulated in petaloid appendage compared with those in anther. GO enrichment analysis indicated that the function of cytokinin is more related to cell differentiation in anther, while auxin is more to cell division in petaloid appendage. B- and C-class floral homeotic genes were expressed in these two androecium parts. Most of the class III HD-ZIP family members, which specify adaxial identity, were expressed lower in petaloid appendage than in anther; while KANADIs and YABBYs, which promote abaxial identity, exhibited opposite expression patterns. In situ hybridization showed that the adaxial marker gene was mainly expressed in the region between the two protrusions of the anther, while the abaxial marker was mainly expressed in petaloid appendage. We hypothesize that the adaxial–abaxial polarity participates in the distinctive anther–petaloid appendage patterning within the asymmetric FS of Canna indica.

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Acknowledgements

This work is supported by Guangdong Natural Science Foundation (Grant No. 2015A030313795), the General Program of National Natural Science Foundation of China (Grant No. 31470410), and National Science and Technology Infrastructure Program of China (Grant No. 2015FY210100).

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Affiliations

  1. Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Xingke Road 723, Tianhe District, Guangzhou, 510650, China

    Xueyi Tian, Pu Zou, Mingzhi Miao, Zulin Ning & Jingping Liao

  2. Guiyang University, Jianlongdong Road 103, Nanming District, Guiyang, 550005, China

    Mingzhi Miao

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  1. Xueyi Tian
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  2. Pu Zou
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  3. Mingzhi Miao
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  4. Zulin Ning
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Contributions

XT performed the experiments (except the SEM observation), and PZ and MM performed SEM observation. XT analyzed the data and wrote the manuscript. ZN and JL designed the research. All authors contributed to the manuscript revisions.

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Correspondence to Zulin Ning or Jingping Liao.

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Communicated by S. Hohmann.

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Tian, X., Zou, P., Miao, M. et al. RNA-Seq analysis reveals the distinctive adaxial–abaxial polarity in the asymmetric one-theca stamen of Canna indica . Mol Genet Genomics 293, 391–400 (2018). https://doi.org/10.1007/s00438-017-1392-3

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Keywords

  • RNA-Seq
  • Adaxial–abaxial polarity
  • Canna indica
  • Petaloid stamen
  • Zingiberales
  • ABC model