Molecular Genetics and Genomics

, Volume 292, Issue 2, pp 475–481 | Cite as

Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast

  • Takafumi Shimasaki
  • Hokuto Ohtsuka
  • Chikako Naito
  • Kenko Azuma
  • Takeshi Tenno
  • Hidekazu Hiroaki
  • Hiroshi Murakami
  • Hirofumi Aiba
Original Article

Abstract

Overexpression of Ecl1-family genes (ecl1+, ecl2+, and ecl3+) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins.

Keywords

Zinc Ecl1 Fission yeast Schizosaccharomyces pombe Life span 

Supplementary material

438_2016_1285_MOESM1_ESM.pdf (23 kb)
Supplementary material 1 (PDF 23 KB)
438_2016_1285_MOESM2_ESM.docx (11 kb)
Supplementary material 2 (DOCX 12 KB)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2017

Authors and Affiliations

  1. 1.Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical SciencesNagoya UniversityNagoyaJapan
  2. 2.Laboratory of Molecular Microbiology, School of AgricultureNagoya UniversityNagoyaJapan
  3. 3.Laboratory of Structural Molecular Pharmacology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical SciencesNagoya UniversityNagoyaJapan
  4. 4.Department of Biological Science, Faculty of Science and EngineeringChuo UniversityTokyoJapan

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