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Molecular Genetics and Genomics

, Volume 292, Issue 2, pp 271–281 | Cite as

Molecular characterization and functional analysis of the OsPsbR gene family in rice

  • Lihua Li
  • Taozhi Ye
  • Xiaoling Gao
  • Rongjun Chen
  • Jinghong Xu
  • Chen Xie
  • Jianqing Zhu
  • Xiaojian Deng
  • Pingrong Wang
  • Zhengjun XuEmail author
Original Article

Abstract

Low temperature may exert a negative impact on agronomical productivity. PsbR was known as the 10 kDa Photosystem II polypeptide. Although plant PsbR is thought to play important roles in photosynthesis, little is known about the contribution of plant PsbR to abiotic stress resistance. The expression patterns of three OsPsbR gene family members, OsPsbR1, OsPsbR2, and OsPsbR3, were characterized in rice ‘Nipponbare’. Under normal condition, OsPsbR1 and OsPsbR3 showed tissue-specific expression, while the expression of OsPsbR2 could not be detected in all tested tissues. OsPsbR1 was upregulated in response to cold stress, and downregulated under drought, salt, or heat conditions. The upregulation of OsPsbR3 was observed under the treatment of ABA, and its downregulation was detected under drought or heat conditions. Upregulation of OsPsbR1 in rice resulted in significantly increased resistance to cold, but did not affect the yield of rice. Furthermore, after 8 h cold-stress treatment, the expression levels of three cold stress-induced marker genes were significantly higher in the overexpression lines L11 and L19 in comparison with the wild type. All these results suggest that OsPsbR1 may play key roles in photosynthesis and cold stress response and thus has the potential to improve cold stress tolerance of crops.

Keywords

Rice Cold OsPsbR gene Expression level Stress Tolerance 

Abbreviations

ROS

Reactive oxygen species

ABA

Abscisic acid

GA

Gibberellin

IAA

Indole-3-acetic acid

WT

Wild type

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant No. 31271802) and the Key Research Project of Sichuan Education Department (Grant No. 13ZA0249).

Compliance with ethical standards

Conflict of interest

Lihua Li declares that he has no conflict of interest. Taozhi Ye declares that he has no conflict of interest. Xiaoling Gao declares that she has no conflict of interest. Rongjun Chen declares that he has no conflict of interest. Jinghong Xu declares that he has no conflict of interest. Chen Xie declares that he has no conflict of interest. Jianqing Zhu declares that he has no conflict of interest. Xiaojian Deng declares that he has no conflict of interest. Pingrong Wang declares that she has no conflict of interest. Zhengjun Xu declares that he has no conflict of interest.

Ethical approval

This article does not contain any studies with human participants performed by any of the authors.

Supplementary material

438_2016_1273_MOESM1_ESM.doc (1.3 mb)
Supplementary material 1 (DOC 1371 kb)

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Copyright information

© Springer-Verlag Berlin Heidelberg 2016

Authors and Affiliations

  • Lihua Li
    • 1
  • Taozhi Ye
    • 1
  • Xiaoling Gao
    • 1
  • Rongjun Chen
    • 1
  • Jinghong Xu
    • 2
  • Chen Xie
    • 3
  • Jianqing Zhu
    • 1
  • Xiaojian Deng
    • 1
  • Pingrong Wang
    • 1
  • Zhengjun Xu
    • 1
    Email author
  1. 1.Key Laboratory of Southwest Crop Genetic Resources and Improvement, Ministry of EducationRice Institute of Sichuan Agricultural UniversityChengduChina
  2. 2.Crop Research InstituteAcademy of Agricultural and Forestry SciencesChengduChina
  3. 3.College of Chemistry and Life ScienceChengdu Normal UniversityChengduChina

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