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High-resolution genetic linkage mapping, high-temperature tolerance and growth-related quantitative trait locus (QTL) identification in Marsupenaeus japonicus

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Abstract

The Kuruma prawn, Marsupenaeus japonicus, is one of the most promising marine invertebrates in the industry in Asia, Europe and Australia. However, the increasing global temperatures result in considerable economic losses in M. japonicus farming. In the present study, to select genetically improved animals for the sustainable development of the Kuruma prawn industry, a high-resolution genetic linkage map and quantitative trait locus (QTL) identification were performed using the RAD technology. The maternal map contained 5849 SNP markers and spanned 3127.23 cM, with an average marker interval of 0.535 cM. Instead, the paternal map contained 3927 SNP markers and spanned 3326.19 cM, with an average marker interval of 0.847 cM. The consensus map contained 9289 SNP markers and spanned 3610.90 cM, with an average marker interval of 0.388 cM and coverage of 99.06 % of the genome. The markers were grouped into 41 linkage groups in the maps. Significantly, negative correlation was detected between high-temperature tolerance (UTT) and body weight (BW). The QTL mapping revealed 129 significant QTL loci for UTT and four significant QTL loci for BW at the genome-wide significance threshold. Among these QTLs, 129 overlapped with linked SNPs, and the remaining four were located in regions between contiguous SNPs. They explained the total phenotypic variance ranging from 8.9 to 12.4 %. Because of a significantly negative correlation between growth and high-temperature tolerance, we demonstrate that this high-resolution linkage map and QTLs would be useful for further marker-assisted selection in the genetic improvement of M. japonicus.

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Acknowledgments

This work was supported by grants of National Hi-Tech R&D program of China (2012AA10A409) and the Taishan Scholar Program For Seed Industry.

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    Authors and Affiliations

    1. Key Laboratory of Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao, 266071, China

      Xia Lu, Sheng Luan, Long Yang Hu & Jie Kong

    2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

      Xia Lu, Sheng Luan, Long Yang Hu & Jie Kong

    3. College of Ocean and Earth Sciences, Xiamen University, Xiamen, 361005, China

      Yong Mao & Sheng Ping Zhong

    4. Shanghai LingEn Biological Technology Co., LTD, Shanghai, 201800, China

      Ye Tao

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    1. Xia Lu
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    Correspondence to Jie Kong.

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    This work was funded by the grants of National Hi-Tech R&D program of China (2012AA10A409) and the Taishan Scholar Program For Seed Industry.

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    This article does not contain any studies with human participants or animals performed by the authors.

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    Communicated by S. Hohmann.

    X. Lu and S. Luan: co-first authors, contributed equally to this work.

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    Lu, X., Luan, S., Hu, L.Y. et al. High-resolution genetic linkage mapping, high-temperature tolerance and growth-related quantitative trait locus (QTL) identification in Marsupenaeus japonicus . Mol Genet Genomics 291, 1391–1405 (2016). https://doi.org/10.1007/s00438-016-1192-1

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