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Transcriptome analysis of BmN cells following over-expression of BmSTAT

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Abstract

The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway are involved in immune response, cell proliferation, differentiation, cell migration and apoptosis. In order to better understand the role of the JAK/STAT pathway in insects we chose Bombyx mori as an experimental model system. Over-expression of BmSTAT in a BmN cell line increased the number of cells in the G2 phase of the cell cycle. Genome-wide transcriptome analysis was performed to identify genes that were differentially expressed following BmSTAT overexpression. Transcriptome data showed that 10,853 and 10,129 expressed genes were obtained from the normal BmN cells and transformed cells, respectively. A total of 800 differentially expressed genes (DEGs) were detected, of which 787 were up-regulated and 13 were down-regulated with T test. In case of FC-test, 252 DEGs were detected, and 123 were expressed in the transformed cells and remaining were in the normal cells. Gene ontology (GO) annotation predicted a functional role for DEGs in catalytic activity, binding, transport, biological regulation, cellular and metabolic processes and pigmentation, while Kyoto encyclopedia of genes and genomes (KEGG) analysis revealed the affected genes to be involved in a multitude of cell signaling pathways. Our findings implicate JAK/STAT signaling in regulating the cell cycle in Bombyx mori, probably in combination with other pathways. These findings justify further investigation into the functional role of the BmSTAT gene.

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Abbreviations

JAK/STAT:

Janus kinase/signal transducers and activators of transcription

DEGs:

Differentially expressed genes

GO:

Gene ontology

KEGG:

Kyoto encyclopedia of genes and genomes

MAPK:

Mitogen-activated protein kinases

FoxO:

Forkhead box O

PI3-AKt:

Phosphatidylinositol 3 kinase-v-akt murine thymoma viral oncogene homolog

mTOR:

Mammalian target of rapamycin

BmHOP:

Tyrosine kinases

BmSOCS :

Cytokine signaling

BmDRK :

Receptor kinases

Bmken :

Ken and barbie

BmPIAS :

Protein inhibitor of activated STAT

BmPI3K :

Phosphatidylinositol-3-kinase

Upd:

Drosophila unpaired

qPCR:

Real-time quantitative PCR

cDNA:

Complementary DNA

FDR:

False discovery rate

GFP:

Green fluorescent protein

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Acknowledgments

This study was funded by the Specialized Research Fund for the Doctoral Program of Higher Education (20113201130002), the National Basic Research Program of China (973 Program, 2012CB114600), the National Natural Science Foundation of China (31072085), Jiangsu Provincial Natural Science Foundation (BK20140324), China Postdoctoral Science Foundation (2014M550306), Jiangsu Planned Projects for Postdoctoral Research Funds (1401151C) and a project funded by the Priority Academic Program of Development of Jiangsu Higher Education Institutions.

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The authors declare that they have no conflicts of interest.

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    Authors and Affiliations

    1. School of Biology and Basic Medical Science, Soochow University, Suzhou, 215123, People’s Republic of China

      Xiaolong Hu, Xi Zhang, Jian Wang, Moli Huang, Renyu Xue, Guangli Cao & Chengliang Gong

    2. National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, People’s Republic of China

      Xiaolong Hu, Renyu Xue, Guangli Cao & Chengliang Gong

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    1. Xiaolong Hu
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    2. Xi Zhang
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    3. Jian Wang
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    7. Chengliang Gong
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    Correspondence to Chengliang Gong.

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    Communicated by Q. Xia.

    The authors X. Hu and X. Zhang have contributed equally to this work.

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    Hu, X., Zhang, X., Wang, J. et al. Transcriptome analysis of BmN cells following over-expression of BmSTAT . Mol Genet Genomics 290, 2137–2146 (2015). https://doi.org/10.1007/s00438-015-1065-z

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