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Molecular characterization of a wheat–Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust

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Abstract

We characterized a wheat–Psathyrostachys huashanica derived line 3-6-4-1 based on genomic in situ hybridization (GISH), molecular marker analysis, and agronomic trait evaluations. The GISH investigations showed that the 3-6-4-1 contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The homoeologous relationships of the introduced P. huashanica chromosomes were determined using EST-STS multiple loci markers from seven wheat homoeologous groups in the parents and the addition line. Twelve EST-STS markers located on the homoeologous group 2 chromosomes of wheat amplified polymorphic bands in 3-6-4-1, which were unique to P. huashanica. An introduced Ns chromosome pair that belonged to homoeologous group 2 was identified using chromosome-specific markers. Inoculation with isolates of the stripe rust pathotypes, CYR31, CYR32, and SY11-14, and mixed races (CYR31, CYR32, and SY11-14) in the seeding and adult stage, respectively, showed that 3-6-4-1 was generally resistant to stripe rust, which was probably attributable to its P. huashanica parent. We also compared a complete set of wheat–P. huashanica disomic addition lines (1Ns–7Ns, 2n = 44 = 22II) to assess their agronomic traits and morphological characteristics, which showed that 3-6-4-1 had improved spike traits compared with its parents. The P. huashanica 2Ns chromosome-specific molecular markers in 3-6-4-1 could be useful for marker-assisted selection in breeding programs to combat stripe rust. This line can be used as a donor source to introduce novel excellent genes from P. huashanica into wheat to widen its genetic diversity, thereby providing new germplasms for wheat breeding.

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Acknowledgments

Much appreciated financial support was provided by the Ministry of Agriculture “948” project of the People’s Republic of China (No. 2013-Z28), the Shaanxi Natural Science Foundation (No. 2012JM3001, No. 2013JZ007), and the Tang Zhong-Ying Breeding Funding Project of the Northwest A&F University. The authors would like to thank Dr. Duncan E. Jackson for useful advice and English language editing of the manuscript.

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Authors and Affiliations

  1. Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Wanli Du, Jing Wang, Liangming Wang, Jun Wu, Jixin Zhao, Shuhui Liu, Qunhui Yang & Xinhong Chen

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  1. Wanli Du
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  2. Jing Wang
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  3. Liangming Wang
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  4. Jun Wu
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  5. Jixin Zhao
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  6. Shuhui Liu
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  7. Qunhui Yang
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  8. Xinhong Chen
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Correspondence to Xinhong Chen.

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Communicated by S. Hohmann.

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Du, W., Wang, J., Wang, L. et al. Molecular characterization of a wheat–Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust. Mol Genet Genomics 289, 735–743 (2014). https://doi.org/10.1007/s00438-014-0844-2

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  • DOI: https://doi.org/10.1007/s00438-014-0844-2

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