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Identification of promoter motifs regulating ZmeIF4E expression level involved in maize rough dwarf disease resistance in maize (Zea Mays L.)

Molecular Genetics and Genomics volume 288, pages 89–99 (2013)Cite this article

Abstract

Maize rough dwarf disease (MRDD, a viral disease) results in significant grain yield losses, while genetic basis of which is largely unknown. Based on comparative genomics, eukaryotic translation initiation factor 4E (eIF4E) was considered as a candidate gene for MRDD resistance, validation of which will help to understand the possible genetic mechanism of this disease. ZmeIF4E (orthologs of eIF4E gene in maize) encodes a protein of 218 amino acids, harboring five exons and no variation in the cDNA sequence is identified between the resistant inbred line, X178 and susceptible one, Ye478. ZmeIF4E expression was different in the two lines plants treated with three plant hormones, ethylene, salicylic acid, and jasmonates at V3 developmental stage, suggesting that ZmeIF4E is more likely to be involved in the regulation of defense gene expression and induction of local and systemic resistance. Moreover, four cis-acting elements related to plant defense responses, including DOFCOREZM, EECCRCAH1, GT1GAMSCAM4, and GT1CONSENSUS were detected in ZmeIF4E promoter for harboring sequence variation in the two lines. Association analysis with 163 inbred lines revealed that one SNP in EECCRCAH1 is significantly associated with CSI of MRDD in two environments, which explained 3.33 and 9.04 % of phenotypic variation, respectively. Meanwhile, one SNP in GT-1 motif was found to affect MRDD resistance only in one of the two environments, which explained 5.17 % of phenotypic variation. Collectively, regulatory motifs respectively harboring the two significant SNPs in ZmeIF4E promoter could be involved in the defense process of maize after viral infection. These results contribute to understand maize defense mechanisms against maize rough dwarf virus.

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Acknowledgments

This study is jointly funded by National Hi-Tech Research Program (2012AA101104), and National Natural Science Foundation of China (30771350).

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Affiliations

  1. National Key Facility of Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 12 Zhong-guan-cun South St, 100081, Beijing, People’s Republic of China

    Liyu Shi, Jianfeng Weng, Changlin Liu, Xinyuan Song, Zhuanfang Hao, Chuanxiao Xie, Mingshun Li, Degui Zhang, Li Bai, Xinhai Li & Shihuang Zhang

  2. Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, 071000, Baoding, People’s Republic of China

    Hongqin Miao

  3. Maize Research Institute, Sichuan Agricultural University, 625014, Ya’an, People’s Republic of China

    Guangtang Pan

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  1. Liyu Shi
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  2. Jianfeng Weng
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  3. Changlin Liu
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  4. Xinyuan Song
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  5. Hongqin Miao
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  6. Zhuanfang Hao
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  7. Chuanxiao Xie
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  8. Mingshun Li
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  9. Degui Zhang
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  10. Li Bai
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  11. Guangtang Pan
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  12. Xinhai Li
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  13. Shihuang Zhang
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Corresponding authors

Correspondence to Xinhai Li or Shihuang Zhang.

Additional information

L. Shi and J. Weng contributed equally to this article.

Sequence data of ZmeIF4E in this article are available in the GenBank data libraries with accession number GU723473.

Communicated by A. K. Tyagi.

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Shi, L., Weng, J., Liu, C. et al. Identification of promoter motifs regulating ZmeIF4E expression level involved in maize rough dwarf disease resistance in maize (Zea Mays L.). Mol Genet Genomics 288, 89–99 (2013). https://doi.org/10.1007/s00438-013-0737-9

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  • DOI: https://doi.org/10.1007/s00438-013-0737-9

Keywords

  • Maize rough dwarf virus
  • eIF4E
  • Association analysis
  • Hormone
  • qRT-PCR