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Molecular Genetics and Genomics

, Volume 283, Issue 2, pp 147–156 | Cite as

Null mutations in Drosophila Optomotor-blind affect T-domain residues conserved in all Tbx proteins

  • Aditya Sen
  • Christian Gadomski
  • Jürgen Balles
  • Yasmin Abassi
  • Christian Dorner
  • Gert O. PflugfelderEmail author
Original Paper

Abstract

The T-box transcription factors TBX2 and TBX3 are overexpressed in many human cancers raising the need for a thorough understanding of the cellular function of these proteins. In Drosophila, there is one corresponding ortholog, Optomotor-blind (Omb). Currently, only two missense mutations are known for the two human proteins. Making use of the developmental defects caused by inactivation of omb, we have isolated and molecularly characterized four new omb mutations, three of them are missense mutations of amino acids fully conserved in all Tbx proteins. We interpret the functional defects in the framework of the known structure of the human TBX3 protein and provide evidence for loss of Omb DNA-binding activity in all three newly identified missense mutations.

Keywords

T-box (Tbx) optomotor-blind Mutation Missense Protein structure DNA binding Drosophila 

Notes

Acknowledgments

We thank the Bloomington Stock Center for Drosophila stocks, E. Jost for fly care, and Deutsche Forschungsgemeinschaft for grant Pf163/14-1.

Supplementary material

438_2009_505_MOESM1_ESM.doc (48 kb)
Supplementary material 1 (DOC 47 kb)

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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Aditya Sen
    • 1
    • 3
  • Christian Gadomski
    • 1
  • Jürgen Balles
    • 2
  • Yasmin Abassi
    • 1
  • Christian Dorner
    • 1
  • Gert O. Pflugfelder
    • 1
    • 2
    Email author
  1. 1.Institute of GeneticsJohannes Gutenberg-UniversitätMainzGermany
  2. 2.Theodor Boveri-InstituteUniversität WürzburgWürzburgGermany
  3. 3.Department of Biochemistry and Molecular BiologyUniformed Services UniversityBethesdaUSA

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