Abstract
Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents—the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.
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Acknowledgments
This work was supported by grants from the Israel Science Foundation (ISF) and the US–Israel Binational Science Foundation (BSF). M. Xaver was supported by grants F3405 and I031-B of the Austrian science foundation.
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Communicated by A. Aguilera.
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Klutstein, M., Xaver, M., Shemesh, R. et al. Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae . Mol Genet Genomics 282, 453–462 (2009). https://doi.org/10.1007/s00438-009-0477-z
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DOI: https://doi.org/10.1007/s00438-009-0477-z