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Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley

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Abstract

Gametophytic self-incompatibility (GSI) in the grasses is controlled by a distinct two-locus genetic system governed by the multiallelic loci S and Z. We have employed diploid Hordeum bulbosum as a model species for identifying the self-incompatibility (SI) genes and for elucidating the molecular mechanisms of the two-locus SI system in the grasses. In this study, we attempted to identify S haplotype-specific cDNAs expressed in pistils and anthers at the flowering stage in H. bulbosum, using the AFLP-based mRNA fingerprinting (AMF, also called cDNA-AFLP) technique. We used the AMF-derived DNA clones as markers for fine mapping of the S locus, and found that the locus resided in a chromosomal region displaying remarkable suppression of recombination, encompassing a large physical region. Furthermore, we identified three AMF-derived markers displaying complete linkage to the S locus, although they showed no significant homology with genes of known functions. Two of these markers showed expression patterns that were specific to the reproductive organs (pistil or anther), suggesting that they could be potential candidates for the S gene.

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Acknowledgments

We thank Drs T. Makino and M. Furusho for providing the H. bulbosum plants (SH7 and SH10) used as parental strains for generating segregating populations. This work was supported by Grant-in Aids for Scientific Research C (Nos. 12660005 and 15580006) from the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT) to K.K.

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Authors and Affiliations

  1. Graduate School of Bioresources, Mie University, Tsu, 514-8507, Japan

    Katsuyuki Kakeda, Toshiro Ibuki, Junko Suzuki, Hidetaka Tadano, Yuko Kurita, Yosuke Hanai & Yasuo Kowyama

Authors
  1. Katsuyuki Kakeda
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  2. Toshiro Ibuki
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  3. Junko Suzuki
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  4. Hidetaka Tadano
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  5. Yuko Kurita
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  6. Yosuke Hanai
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  7. Yasuo Kowyama
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Corresponding author

Correspondence to Katsuyuki Kakeda.

Additional information

Communicated by S. Hohmann.

Nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession numbers AB455978–AB455990.

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438_2008_383_MOESM1_ESM.TIF.tif

S mapping population 1 (SMP1). a S and Z genotypes of theparents and their gametes. The S genotype of all progeny must be S1S2 because onlyS2Z2 pollen can effect fertilization on the pistil with the genotype S1.1 Z2.3. The Zgenotypes are expected to segregate as Z2.2 and Z2.3 in a ratio of 1:1. b Detection ofrecombinants between S and an adjacent marker locus, designated as M (for example,HTL in this study). Assuming that two marker alleles M1 and M2 are located adjacent tothe S1 and S2 alleles, respectively, in the pollen parent, only the S2M1 and S2M2 pollencan effect fertilization because S1 pollen is incompatible. The S2M1 gamete resultsfrom a recombination between the S and M loci. Thus, one can distinguish betweenrecombinants (M1M1) and non-recombinants (M1M2) by checking the marker genotypes only. We also generated the ZMP1 population by the reciprocal cross(S1.2Z2.2 x S1.1Z2.3) to detect recombinants between Z and an adjacent marker locus(bcd266). In this case, the marker genotype M2M2 represented the recombinant whileM2M3 were non-recombinants. (TIFF 169 kb)

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Kakeda, K., Ibuki, T., Suzuki, J. et al. Molecular and genetic characterization of the S locus in Hordeum bulbosum L., a wild self-incompatible species related to cultivated barley. Mol Genet Genomics 280, 509–519 (2008). https://doi.org/10.1007/s00438-008-0383-9

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