Abstract
A new genus Douglasdeweya containing the two species, Douglasdeweya deweyi and D. wangii was published in 2005 by Yen et al. based upon the results of cytogenetical and morphological findings. The genome constitution of Douglasdeweya—PPStSt—allowed its segregation from the genus Pseudoroegneria which contains the StSt or StStStSt genomes. Our previous work had demonstrated the utility of using 5S rDNA units, especially the non-transcribed spacer sequence variation, for the resolution of genomes (haplomes) previously established by cytology. Here, we show that sequence analysis of the 5S DNA units from these species strongly supports the proposed species relationships of Yen et al. (Can J Bot 83:413–419, 2005), i.e., the PP genome from Agropyron and the StSt genome from Pseudoroegneria. Analysis of the 5S rDNA units constitutes a powerful tool for genomic research especially in the Triticeae.
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Acknowledgments
We thank Richard R.-C. Wang, USDA-ARS, Forage and Range Research Laboratory, Utah State University, 695 North 1100 East, Logan, UT, 84322-6300, USA, for seed material. We express our thanks to Mr. Eric Ouellet for technical assistance and not least we are indebted to Dr. S. Molnar, Agriculture and Agri-Food Canada, Ottawa for comments on a draft of this manuscript.
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Baum, B.R., Johnson, D.A. Molecular confirmation of the genomic constitution of Douglasdeweya (Triticeae: Poaceae): demonstration of the utility of the 5S rDNA sequence as a tool for haplome identification. Mol Genet Genomics 279, 621–628 (2008). https://doi.org/10.1007/s00438-008-0338-1
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DOI: https://doi.org/10.1007/s00438-008-0338-1